Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network
Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network
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DOI：
10.1093/embo-reports/kve061
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发表时间：
2001-04-01
期刊：
影响因子：
7.7
通讯作者：
Yoshimori, T
中科院分区：
文献类型：
--
作者：
Kihara, A;Kabeya, Y;Yoshimori, T
自噬是细胞内大量蛋白质降解系统。已知Beclin参与了这一过程，但其作用尚不清楚。在这项研究中，我们发现Beclin与磷脂酰肌醇（PtdIns）3-激酶共免疫沉淀，这也是自噬所需的，这表明Beclin是PtdIns 3-激酶复合物的一个组成部分。使用交联剂的定量分析表明，所有Beclin与PtdIns 3-激酶形成复合物，而类似于50%的PtdIns 3-激酶保持不含Beclin。间接免疫荧光显微镜显示，大多数Beclin和PtdIns 3-激酶定位于trans-Golgi网络（TGN）。一些PtdIns 3-激酶也分布在晚期内体中。这些结果表明，Beclin和PtdIns 3-激酶控制自噬作为一个复杂的TGN。
Autophagy is an intracellular bulk protein degradation system. Beclin is known to be involved in this process; however, its role is unclear. In this study, we showed that Beclin was co-immunoprecipitated with phosphatidylinositol (PtdIns) 3-kinase, which is also required for autophagy, suggesting that Beclin is a component of the PtdIns 3-kinase complex. Quantitative analyses using a cross-linker showed that all Beclin forms a complex with PtdIns 3-kinase, whereas similar to 50% of PtdIns 3-kinase remains free from Beclin. Indirect immunofluorescence microscopy demonstrated that the majority of Beclin and PtdIns 3-kinase localize to the trans-Golgi network (TGN). Some PtdIns 3-kinase is also distributed in the late endosome. These results suggest that Beclin and PtdIns 3-kinase control autophagy as a complex at the TGN.