Physical mapping of potassium channel gene clusters on mouse chromosomes three and six.
Physical mapping of potassium channel gene clusters on mouse chromosomes three and six.
复制标题
小鼠第三和第六号染色体上钾通道基因簇的物理图谱。
DOI：
10.1006/geno.1997.4799
复制
发表时间：
1997
期刊：
影响因子：
4.4
通讯作者：
Tempel,BL
中科院分区：
文献类型：
--
作者：
Street,VA;Tempel,BL
根据与果蝇中相关基因的序列同一性和相似性，哺乳动物电压门控 K 通道基因被分为四个亚家族（Shaker、Shab、Shal 和 Shaw）。电压门控 K 通道基因的遗传图谱表明，小鼠 Chr 3 和 6 上存在类似的多基因簇，并表明这些簇可能是通过染色体复制产生的。在本报告中，使用限制性内切酶和酵母染色体断裂方法构建了基于 YAC 的聚集型小鼠 Shaker 样 K 通道基因的物理图谱。这些数据将 Chr 3 上的物理间距定义为 5′-Kcna3-(60 kb)-Kcna2-(90 kb)-Kcna8-3′，在 Chr 6 上定义为 5′-Kcna6-(80 kb)-Kcna1-(110 kb)-Kcna5-3′，所有基因在各自的簇内以头尾相连的方式定向。这些 K 通道基因簇的详细物理图谱为古代基因组四倍体化事件的想法提供了额外的支持。
Mammalian voltage-gated K channel genes have been divided into four subfamilies (Shaker, Shab, Shal, and Shaw) based on their sequence identity and similarity to related genes inDrosophila.Genetic mapping of the voltage-gated K channel genes has shown that similar multigene clusters exist on mouse Chr 3 and 6 and suggests that the clusters may have arisen through chromosomal duplication. In this report, YAC-based physical maps of the clustered mouse Shaker-like K channel genes have been constructed using restriction endonuclease and yeast chromosome fragmentation approaches. These data define the physical spacing as 5′–Kcna3–(60 kb)–Kcna2–(90 kb)–Kcna8– 3′ on Chr 3, and as 5′–Kcna6–(80 kb)–Kcna1–(110 kb)–Kcna5–3′ on Chr 6, with all genes oriented in a head-to-tail manner within their respective clusters. These detailed physical maps of both K channel gene clusters provide additional support for the idea of an ancient genome tetraploidization event.