HUMAN BONE-MARROW MICROVASCULAR ENDOTHELIAL-CELLS SUPPORT LONG-TERM PROLIFERATION AND DIFFERENTIATION OF MYELOID AND MEGAKARYOCYTIC PROGENITORS
HUMAN BONE-MARROW MICROVASCULAR ENDOTHELIAL-CELLS SUPPORT LONG-TERM PROLIFERATION AND DIFFERENTIATION OF MYELOID AND MEGAKARYOCYTIC PROGENITORS
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DOI：
10.1182/blood.v86.9.3353.bloodjournal8693353
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发表时间：
1995-11-01
期刊：
影响因子：
20.3
通讯作者：
ASCH, AS
中科院分区：
文献类型：
--
作者：
RAFII, S;SHAPIRO, F;ASCH, AS
内皮细胞是骨髓（BM）微环境的主要组成部分，其调节造血祖细胞和干细胞的运输和归巢。在本文中，我们提供的证据表明，骨髓内皮细胞（BMEC）也支持多谱系造血细胞的可溶性细胞因子的阐述，造血祖细胞与BMEC单层直接接触孵育，或物理分离的微孔膜，扩大5倍至7倍，在7天，在没有外源性细胞因子。在BMEC单层存在下生长的增殖祖细胞的流式细胞术分析显示，到共培养的第14天，70%至80%的造血细胞是髓样的，表达CD 15或CD 14，14%至19%是巨核细胞，表达GPIIb/IIIa或GPIb。在transwell培养板的上室中培养的来自脐带血的CD 34（+）细胞，以及与BMEC单层直接接触生长的细胞，产生祖细胞长达70天。未受刺激的BMEC单层组成性产生白细胞介素-6、Kit-配体、粒细胞集落刺激因子和粒细胞巨噬细胞集落刺激因子。这些数据表明，BMEC调节造血祖细胞和长期培养起始细胞的增殖的谱系特异性细胞因子的阐述。(C)1995年，美国血液学会。
Endothelial cells are a major component of the bone marrow (BM) microenvironment that regulate the trafficking and homing of hematopoietic progenitor and stem cells. In this paper, we provide evidence that BM endothelial cells (BMECs) also support multilineage hematopoiesis by elaboration of soluble cytokines, Hematopoietic progenitor cells incubated in direct contact with BMEC monolayers, or physically separated by microporous membrane, expanded fivefold to sevenfold at 7 days, in the absence of exogenous cytokines. Flow cytometric analysis of proliferating progenitor cells grown in the presence of BMEC monolayers showed that by day 14 of coculture, 70% to 80% of hematopoietic cells were myeloid, expressing CD15 or CD14, and 14% to 19% were megakaryocytic, expressing GPIIb/IIIa or GPIb. CD34(+) cells derived from umbilical cord blood, cultured in the upper chamber of transwell culture plates, as well as the cells grown in direct contact with BMEC monolayers, generated progenitors for up to 70 days. Unstimulated BMEC monolayers constitutively produce interleukin-6, Kit-ligand, granulocyte colony-stimulating factor, and granulocyte macrophage colony-stimulating factor. These data suggest that BMEC regulate proliferation of hematopoietic progenitor cells and long-term culture initiating cells by elaboration of lineage-specific cytokines. (C) 1995 by The American Society of Hematology.