Reevaluation of Two Quantitative Trait Loci for Type II Resistance to Fusarium Head Blight in Wheat Germplasm PI 672538
Reevaluation of Two Quantitative Trait Loci for Type II Resistance to Fusarium Head Blight in Wheat Germplasm PI 672538
复制标题
小麦种质 PI 672538 中抗镰刀菌赤霉病 II 型的两个数量性状位点的重新评估
DOI：
10.1094/phyto-04-16-0170-r
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发表时间：
2017-01-01
期刊：
影响因子：
3.2
通讯作者：
Luo, P. G.
中科院分区：
文献类型：
--
作者：
Li, X.;Xiang, Z. P.;Luo, P. G.
赤霉病（Fusarium head blight，FHB）是小麦的一种毁灭性病害，主要由禾谷镰刀菌（Fusarium graminearum）引起。由源自杂交 PI 672538 x L661 的 229 株 F-2 和 F-2:3 植物组成的群体用于评估对 FHB 的反应。 F2群体中的FHB抗性数据分布表明，一些数量性状位点（QTL）控制着PI 672538中的FHB抗性。我们通过使用WinQTLCart 2.5软件分析抗性数据和PCR扩增结果，进一步检测到两个主要QTL（Qfhs-2B、Qfhs-3B）。两侧为 Xbarc55-2B 和 Xbarc1155-2B 的 Qfhs-2B，解释了患病小穗 (PDS) 百分比表型变异的 11.6% 以上，而两侧为 Xwmc54-3B 和 Xgwm566-3B 的 Qfhs-3B，解释了患病小穗中 10% 以上的 PDS 表型变异。 F-2:3 人口。此外，Qfhs-3B在谱系、遗传、耐药反应、染色体位置和标记诊断方面与Fhb1不同。我们还在相似的染色体区域检测了其他抗病指标的 QTL，包括受损籽粒的百分比和千粒重。因此，PI 672538的FHB抗性主要由定位在2B (FhbL693a)和3B (FhbL693b)上的两个主要QTL控制。 PI 672538可能是提高小麦FHB抗性的有用种质。
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease in wheat. A population consisting of 229 F-2 and F-2:3 plants derived from the cross PI 672538 x L661 was used to evaluate the reactions to FHB. The FHB resistance data distribution in the F2 population indicates that some quantitative trait loci (QTLs) were controlling the FHB resistance in PI 672538. We further detected two major QTLs (Qfhs-2B, Qfhs-3B) from analysis of the resistance data and the PCR-amplified results using WinQTLCart 2.5 software. Qfhs-2B, flanked by Xbarc55-2B and Xbarc1155-2B, explained more than 11.6% of the phenotypic variation of the percentage of diseased spikelets (PDS), and Qfhs-3B, flanked by Xwmc54-3B and Xgwm566-3B, explained more than 10% of the PDS phenotypic variation in the F-2:3 population. In addition, Qfhs-3B was different from Fhb1 in terms of the pedigree, inheritance, resistance response, chromosomal location, and marker diagnosis. We also detected QTLs for other disease resistance indices, including the percentage of damaged kernels and 1,000-grain weight, in similar chromosomal regions. Therefore, the FHB resistance of PI 672538 was mainly controlled by two major QTLs, mapped on 2B (FhbL693a) and 3B (FhbL693b). PI 672538 could be a useful germplasm for improving wheat FHB resistance.