Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals
Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals
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DOI：
10.1007/s00253-009-2304-1
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发表时间：
2010-03-01
影响因子：
5
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Nishitani, Takashi;Shimada, Mariko;Ueda, Mitsuyoshi
在现代工业社会中，钼是发展稀有金属工业的重要金属之一。从废物中回收稀有金属是很重要的，因为它们在技术上和经济上都很难被挖掘和提纯，而且它们只存在于世界上少数几个地区。在这项研究中，来自大肠杆菌的ModE蛋白，这是一种具有结合可溶性钼形式的钼的能力的钼酸盐依赖性转录调节因子，通过基于α-凝集素的细胞表面展示系统展示在酵母细胞表面，用于吸附和回收钼。显示的ModE，证实了免疫荧光标记，捕捉到更好地在pH值3.0比在碱性pH值。酵母细胞展示的C-末端结构域的ModE，其中缺乏N-末端DNA结合域，更有效地吸附比那些展示全长ModE，这表明删除无关的金属结合的结构域增强的结合能力。我们的研究结果表明，在酵母细胞的细胞表面上的吸附系统展示ModE是有效的，不仅为吸附作为一种稀有金属的生物吸附剂，但也为位于细胞表面上的金属的容易回收。
In modern industrial society, molybdenum is one of the important metals for development of the industry of rare metals. It is important to recycle the rare metals from wastes because they are technically and economically difficult to be dug and be purified, and they exist in only a few regions in the world. In this study, ModE protein derived from Escherichia coli, which is a molybdate-dependent transcriptional regulator with the ability to bind molybdate as a form of soluble molybdenum, was displayed on the yeast cell surface by alpha-agglutinin-based cell surface display system for the adsorption and recovery of molybdate. Displayed ModE, confirmed by immunofluorescence labeling, caught molybdate more preferably at pH 3.0 than at basic pH. Yeast cells displaying C-terminal domain of ModE, which lacks N-terminal DNA binding domain, more effectively adsorbed molybdate than those displaying full-length ModE, suggesting that the deletion of the domain unrelated to metal binding enhanced the binding ability. Our results indicated that the adsorption system on cell surface of yeast cells displaying ModE is effective not only for adsorption of molybdate as a rare metal bioadsorbent but also for the easy recovery of molybdate located on the cell surface.