Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization. Corrigendum.
Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization. Corrigendum.
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Rv0100，结核分枝杆菌中拟议的酰基载体蛋白：表达、纯化和结晶。
DOI：
10.1107/s2053230x2000271x
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Movahedzadeh,Farahnaz
中科院分区：
文献类型：
--
作者：
Bondoc,JasperMarcG;Gutka,HitenJ;Almutairi,MashalM;Patwell,Ryan;Rutter,MaxwellW;Wolf,NinaM;Samudrala,Ram;Mehboob,Shahila;Dementiev,Alexey;Abad-Zapatero,Cele;Movahedzadeh,Farahnaz
酰基载体蛋白是原核生物脂肪酸生物合成的重要组成部分。Rv0100是结核分枝杆菌（Mycobacterium tuberculosis）中的一个重要ACP，是结核病的病原体，因此Rv0100有可能成为一个新的抗结核药物靶点。在这里，成功地克隆和纯化Rv0100使用耻垢分枝杆菌作为宿主的报告。获得纯化蛋白质的晶体，其衍射至1.9 nm的分辨率。 总的来说，这项工作为未来针对这种潜在的新型药物靶点的药物发现和开发奠定了基础。
Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.