Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells
Temporal change in IL-6 mRNA and protein expression produced by cyclic stretching of human pulmonary artery endothelial cells
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DOI：
10.3892/ijmm.2012.1023
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发表时间：
2012-09-01
影响因子：
5.4
通讯作者：
Inaoka, Hidenori
中科院分区：
文献类型：
--
作者：
Kobayashi, Kozue;Tanaka, Masumi;Inaoka, Hidenori
研究了周期性牵张对人肺动脉内皮细胞（HPAECs）白细胞介素（IL）-6基因表达和蛋白质产生的影响。即使在没有拉伸的细胞中，IL-6蛋白也增加。通过将拉伸细胞中的IL-6蛋白水平除以相同取样时间的未拉伸细胞中的IL-6蛋白水平确定的倍数变化表明，拉伸增加了IL-6蛋白。至少1小时的拉伸是必要的，以引起IL-6蛋白的增加，并在拉伸开始后3小时达到峰值。停止牵拉后，IL-6蛋白水平没有进一步升高。IL-6基因的表达水平显着增加拉伸和拉伸开始后30分钟达到峰值。IL-6基因和蛋白表达高峰时间的差异可能反映了IL-6 mRNA出现后蛋白合成的过程。
The time courses of interleukin (IL)-6 gene expression and protein production were examined in human pulmonary artery endothelial cells (HPAECs) subjected to cyclic stretching. IL-6 protein was increased even in cells without stretching. Fold changes determined by dividing the level of IL-6 protein in stretched cells by that in unstretched cells at the same sampling times indicated that IL-6 protein was increased by stretching. At least 1 h of stretching was necessary to elicit an increase of IL-6 protein, and the levels peaked at 3 h after the start of stretching. After withdrawal of stretching, there was no further increase of IL-6 protein. The expression levels of the IL-6 gene were significantly increased by stretching and peaked at 30 min after the start of stretching. The difference in the peak times of IL-6 gene and protein expression likely reflects the process of protein synthesis after the appearance of IL-6 mRNA.