Neutrophil recruitment as a factor limiting injury or promoting recovery from acute lung injury.
Neutrophil recruitment as a factor limiting injury or promoting recovery from acute lung injury.
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中性粒细胞募集是限制损伤或促进急性肺损伤恢复的因素。
DOI：
10.1152/ajpheart.1997.272.1.h279
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Gee,MH
中科院分区：
文献类型：
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作者：
Carey,LA;Perkowski,SZ;Lipsky,CL;Cirelli,RA;SpathJr,JA;Gee,MH
我们研究了 1) 给予内毒素 (10 ng.kg-1.min-1, n = 5) 4 小时的绵羊中的中性粒细胞动员，2) 存活 (n = 17) 和死亡绵羊 (n = 8) 在 12 小时内毒素输注过程中的情况，以及 3) 成年绵羊 (n = 8) 或羔羊 (n = 8) 输注内毒素 12 小时。内毒素处理后 4 小时，绵羊的骨髓细胞从基线值 10,533 +/- 1,784 下降至 5,966 +/- 1,980 个细胞/微升 (P < 0.05)。内毒素输注 12 小时后，循环中性粒细胞仍从基线值 2,000-4,000 个细胞/微升减少至羔羊中的 343 +/- 70 个细胞/微升，以及未存活绵羊中的 484 +/- 236 个细胞/微升，而存活羊中的中性粒细胞开始恢复至 1,838 +/- 467 个细胞/微升。在羔羊和未存活的绵羊中，输注内毒素 12 小时可使肺淋巴蛋白清除率增加十倍，而存活的绵羊则增加五倍。从暴露于羔羊内毒素血浆的绵羊骨髓中培养的中性粒细胞未能增加细胞数量（609 +/- 229至610 +/- 182细胞/微升），而暴露于成年绵羊内毒素后血浆的类似细胞显示细胞数量显着增加（1,069 +/- 101至2,293 +/- 448细胞/微升，P <0.05）。我们的结果与以下假设一致：炎症期间将中性粒细胞募集到循环中的能力对于限制急性肺损伤的严重程度很重要。
We studied 1) neutrophil mobilization in sheep given endotoxin (10 ng.kg-1.min-1, n = 5) for 4 h, 2) surviving (n = 17) and nonsurviving sheep (n = 8) during a 12-h infusion of endotoxin, and 3) adult sheep (n = 8) or lambs (n = 8) infused with endotoxin for 12 h. Bone marrow cells of sheep declined from a baseline value of 10,533 +/- 1,784 to 5,966 +/- 1,980 cells/microliter (P < 0.05) 4 h after endotoxin. After 12 h of endotoxin infusion, circulating neutrophils remained reduced from baseline values of 2,000-4,000 cells/microliter to 343 +/- 70 in lambs and 484 +/- 236 in nonsurviving sheep, while beginning to recover in surviving sheep to 1,838 +/- 467 cells/microliter. In lambs and nonsurviving sheep, a 12-h infusion of endotoxin increased lung lymph protein clearance tenfold compared with a fivefold increase in surviving sheep. Neutrophils cultured from sheep bone marrow exposed to lamb postendotoxin plasma failed to increase in cell number (609 +/- 229 to 610 +/- 182 cells/microliter), whereas similar cells exposed to adult sheep postendotoxic plasma showed a significant increase in cell number (1,069 +/- 101 to 2,293 +/- 448 cells/microliter, P <0.05). Our results are consistent with the hypothesis that the ability to recruit neutrophils to the circulation during periods of inflammation is important in limiting the severity of acute lung injury.