CB1 and CB2 contribute to antinociceptive and anti-inflammatory effects of electroacupuncture on experimental arthritis of the rat temporomandibular joint
CB1 and CB2 contribute to antinociceptive and anti-inflammatory effects of electroacupuncture on experimental arthritis of the rat temporomandibular joint
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DOI：
10.1139/y2012-130
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发表时间：
2012-11-01
影响因子：
2.1
通讯作者：
Vale, Mariana Lima
中科院分区：
文献类型：
--
作者：
Gondim, Delane Viana;Bezerra Araujo, Joana Claudia;Vale, Mariana Lima
据报道，电针（EA）和大麻素在关节炎动物模型中具有抗炎和镇痛作用。雄性 Wistar 大鼠颞下颌关节 (TMJ) 注射生理盐水或酵母聚糖 (2 mg)。给予酵母聚糖后 2 小时或前 1 小时进行 EA（10 Hz，30 分钟）。在 EA 治疗前给予 AM251 或 AM630（3 mg/kg，腹腔注射）。施用酵母聚糖后出现机械性过度伤害感受。给予酵母聚糖后6小时处死大鼠并取出关节进行组织病理学分析。处死 TMJ 关节炎动物后评估 CB1 和 CB2 受体的基因表达。 EA 抑制酵母聚糖诱导的过度伤害感受 (p < 0.05)。 AM251 显着逆转 EA 的抗伤害作用，表明 CB1 受体参与了这种作用。 AM630 逆转了 EA 的抗炎作用。在 EA 治疗组中，酵母聚糖诱导关节炎后 6 小时，CB1 和 CB2 受体基因表达上调。我们观察到与第 6 小时相比，第 24 小时 EA 组的 CB2 受体基因表达下调。与第 6 小时相比，还发现了更高的 CB1 受体基因表达。 EA 产生抗伤害和抗炎作用，这些作用似乎是通过 CB1 和 CB2 受体激活介导的。
Electroacupuncture (EA) and cannabinoids have been reported to have anti-inflammatory and antinociceptive effects in animal models of arthritis. Male Wistar rats were injected with saline or zymosan (2 mg) into the temporomandibular joint (TMJ). EA (10 Hz, 30 min) was performed 2 h after or 1 h before zymosan administration. AM251 or AM630 (3 mg/kg, i.p.) were administered before EA treatment. Mechanical hypernociception was accessed after zymosan administration. Rats were sacrificed 6 h after zymosan administration and the joint was removed for histopathological analysis. The gene expression of CB1 and CB2 receptors was assessed after sacrifice of the TMJ arthritic animals. EA inhibited zymosan-induced hypernociception (p < 0.05). AM251 reversed significantly the antinociceptive effect of EA, suggesting that the CB1 receptor is involved in this effect. AM630 reversed the anti-inflammatory effect of EA. CB1 and CB2 receptor gene expression was upregulated 6 h after zymosan-induced arthritis in the EA-treated group. We observed downregulation of CB2 receptor gene expression in the EA group at the 24th hour compared with the 6th hour. Higher CB1 receptor gene expression was also found compared with the 6th hour. EA produced antinociceptive and anti-inflammatory effects, and these effects appeared to be mediated through CB1 and CB2 receptor activation.