Inhibitory neurones of a motor pattern generator in Xenopus revealed by antibodies to glycine
Inhibitory neurones of a motor pattern generator in Xenopus revealed by antibodies to glycine
复制标题
甘氨酸抗体揭示了非洲爪蟾运动模式发生器的抑制性神经元
作者：
Nicholas Dale;Nicholas Dale;O. P. Ottersen;A. Roberts;Jon Storm
甘氨酸和谷氨酸-氨基丁酸(γ-氨基丁酸)是重要的抑制性递质1，2。虽然谷氨酸脱羧酶(GAD)3，4或GABA5-8的免疫细胞化学方法可以显示以GABA为递质的神经元，但还没有类似的技术可用于选择性地显示甘氨酸能神经元。我们现在已经生产了多克隆抗体，可以识别戊二醛固定组织中的甘氨酸。我们利用这些抗体研究了甘氨酸在非洲爪哇胚胎简单中枢神经系统(CNS)中的分布，在解剖学和生理学上定义了一类相互抑制的神经元间，即连合神经元9-11。这些神经元间在游泳运动模式的产生中起着重要作用，被认为是甘氨酸11-13。甘氨酸抗体对这些神经元进行特异性染色，揭示它们在胚胎CNS中的分布和数量。这是首次证明甘氨酸样免疫反应性选择性定位于一类假定的甘氨酸能神经元，该神经元的生理、药理学和解剖学特征。
Glycine and γ-aminobutyric acid (GABA) are inhibitory transmitters of major importance1,2. Whereas neurones using GABA as the transmitter can be visualized by immunocytochemical methods for glutamate decarboxylase (GAD)3,4 or GABA5–8, no comparable techniques have been available for the selective visualization of glycinergic neurones. We have now produced polyclonal antibodies which specifically recognize glycine in glutaral-dehyde-fixed tissue. We used these antibodies to investigate the distribution of glycine in the simple central nervous system (CNS) of the Xenopus embryo, which contains an anatomically and physiologically defined class of reciprocal inhibitory interneurones, the commissural interneurones9–11. These interneurones have an important role in the generation of the swimming motor pattern and are thought to be glycinergic11–13. The glycine antibodies specifically stain these interneurones, revealing their distribution and number in the embryo CNS. This is the first demonstration of the selective localization of glycine-like immunoreactivity in a putative glycinergic class of neurone that has been characterized physiologically, pharmacologically and anatomically.