Identification and expression analysis of BMP signaling inhibitors genes of the DAN family in amphioxus
Identification and expression analysis of BMP signaling inhibitors genes of the DAN family in amphioxus
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DOI：
10.1016/j.gep.2013.07.005
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发表时间：
2013-12-01
影响因子：
1.2
通讯作者：
Bertrand, Stephanie
中科院分区：
文献类型：
--
作者：
Le Petillon, Yann;Oulion, Silvan;Bertrand, Stephanie
骨形态发生蛋白(BMP)是转化生长因子-β(TGF-β)家族的成员，参与后生动物的许多发育过程，如胚轴规范。它们的各种作用在一定程度上是由阻碍BMPs与其特定受体相互作用的抑制剂控制的。在这里，我们将注意力集中在神经母细胞瘤(DAN)抑制剂家族的差异筛选选择基因Aberrative上。尽管它们在脊椎动物中有很好的特征，但在其他后生动物物种中关于这个家族的数据很少。为了了解这些抑制物在脊索动物中的潜在发育作用的进化，我们在文昌鱼中鉴定了该家族的成员，并研究了它们在胚胎发育过程中的表达模式。我们的数据表明Cerberus/Dand5亚家族基因的功能在脊索动物中是保守的，而Gremlin1/2和NBL1亚家族基因似乎在每个脊索系中获得了不同的表达模式。另一方面，Gremlin在文昌鱼神经板缘的早期表达支持了脊索动物神经板缘基因网络保守的假说。(C)2013爱思唯尔B.V.保留所有权利。
Bone morphogenetic proteins (BMPs) are members of the Transforming Growth Factor-beta (TGF-beta family implicated in many developmental processes in metazoans such as embryo axes specification. Their wide variety of actions is in part controlled by inhibitors that impede the interaction of BMPs with their specific receptors. Here, we focused our attention on the Differential screening-selected gene Aberrative in Neuroblastoma (DAN) family of inhibitors. Although they are well-characterized in vertebrates, few data are available for this family in other metazoan species. In order to understand the evolution of potential developmental roles of these inhibitors in chordates, we identified the members of this family in the cephalochordate amphioxus, and characterized their expression patterns during embryonic development. Our data suggest that the function of Cerberus/Dand5 subfamily genes is conserved among chordates, whereas Gremlin1/2 and NBL1 subfamily genes seem to have acquired divergent expression patterns in each chordate lineage. On the other hand, the expression of Gremlin in the amphioxus neural plate border during early neurulation strengthens the hypothesis of a conserved neural plate border gene network in chordates. (C) 2013 Elsevier B.V. All rights reserved.