Toward a Biomarker of Oxidative Stress: A Fluorescent Probe for Exogenous and Endogenous Malondialdehyde in Living Cells
Toward a Biomarker of Oxidative Stress: A Fluorescent Probe for Exogenous and Endogenous Malondialdehyde in Living Cells
复制标题
氧化应激生物标志物：活细胞中外源性和内源性丙二醛的荧光探针。
DOI：
10.1021/acs.analchem.5b02032
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发表时间：
2015-08-18
影响因子：
7.4
通讯作者：
Liu, Zhihong
中科院分区：
文献类型：
--
作者：
Chen, Jin;Zeng, Lingyu;Liu, Zhihong
丙二醛（MDA）是氧化应激的重要生物标志物。生物系统中MDA水平的变化往往代表着与多种疾病相关的病理变化。尽管已经开发了多种用于 MDA 检测的技术，但在活细胞中探测这种生物标志物仍未得到探索。在此，我们报道了一种具有协同光诱导电子转移（PET）-氢键机制的开启荧光探针MDAP-1，该探针首次实现了生理条件下的MDA传感，具有优异的灵敏度和特异性。该探针对 MDA 的响应具有高达 >170 倍的荧光增强因子 (FEF) 和较大的斯托克斯位移 (∼180 nm)。进一步的生物学评估表明 MDAP-1 能够检测活细胞中的内源性和外源性 MDA。它可用于追踪 H2O2 刺激下氧化应激下 MDA 的生成。我们相信这项工作的结果将有助于未来MDA相关生物学事件的研究以及潜在病理机制的阐明。
Malondialdehyde (MDA) is a significant biomarker of oxidative stress. Variations of MDA level in biological systems often represent pathological changes that are related with many types of diseases. Although a variety of techniques have been developed for MDA detection, the probing of this biomarker in living cells remains unexplored. Herein, we report a turn-on fluorescent probe, MDAP-1, with a synergistic photoinduced electron transfer (PET)-hydrogen bonding mechanism, which for the first time realizes MDA sensing under physiological conditions with excellent sensitivity and specificity. The probe responds to MDA with a fluorescence enhancement factor (FEF) of up to >170-fold and a large Stokes shift (∼180 nm). Further biological evaluations show that MDAP-1 is able to detect both endogenous and exogenous MDA in living cells. It can be used to track the generation of MDA under oxidative stress, as stimulated by H2O2. We believe the results of this work will be helpful to the studies of MDA-related biological events and the elucidation of the underlying pathological mechanism in the future.