The mechanism of signal transduction by two-component systems
The mechanism of signal transduction by two-component systems
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DOI：
10.1016/j.sbi.2010.09.010
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发表时间：
2010-12-01
影响因子：
6.8
通讯作者：
Marina, Alberto
中科院分区：
文献类型：
--
作者：
Casino, Patricia;Rubio, Vicente;Marina, Alberto
由同型二聚体组氨酸激酶 (HK) 和反应调节器 (RR) 组成的双组分系统是细菌中的主要信号转导装置。通常，该信号会触发 HK 在一个 His 残基处进行自磷酸化，然后磷酰基从磷酸-His 转移到 RR 中的 Asp 残基。信号消退经常涉及 HK 磷酸酶活性导致的磷酸-RR 去磷酸化。最近的晶体结构和 HK-RR 复合物的生化实验大大增加了我们对这些反应以及 HK-RR 配对中伴侣特异性决定因素的理解。顺式自磷酸化（一个亚基自身磷酸化）发生在一些 HK 中，而反式自磷酸化发生在其他 HK 中。我们回顾并整合了这些新信息，讨论了三种反应的机制，并提出了这些系统跨膜信号传导的模型。
Two-component systems, composed of a homodimeric histidine kinase (HK) and a response regulator (RR), are major signal transduction devices in bacteria. Typically the signal triggers HK autophosphorylation at one His residue, followed by phosphoryl transfer from the phospho-His to an Asp residue in the RR. Signal extinction frequently involves phospho-RR dephosphorylation by a phosphatase activity of the HK. Our understanding of these reactions and of the determinants of partner specificity among HK-RR couples has been greatly increased by recent crystal structures and biochemical experiments on HK-RR complexes. Cis-autophosphorylation (one subunit phosphorylates itself) occurs in some HKs while trans-autophosphorylation takes place in others. We review and integrate this new information, discuss the mechanism of the three reactions and propose a model for transmembrane signaling by these systems.