Fluorescent protein capped mesoporous nanoparticles for intracellular drug delivery and imaging.
Fluorescent protein capped mesoporous nanoparticles for intracellular drug delivery and imaging.
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DOI：
10.1002/chem.201302026
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Xinjian Yang;Zhenhua Li;M. Li;Jinsong Ren;Xiaogang Qu
中科院分区：
文献类型：
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作者：
Xinjian Yang;Zhenhua Li;M. Li;Jinsong Ren;Xiaogang Qu
利用组氨酸标记的、青色荧光蛋白（CFP）修饰的磁性介孔二氧化硅纳米粒子（MMSNs）构建了一种用于细胞内药物递送和同时荧光成像的多功能系统。这种蛋白质封端的多功能纳米结构具有高度生物相容性，并且不影响细胞活力或增殖。CFP不仅作为封端剂，而且作为荧光成像剂。通过基于组氨酸的置换激活纳米组装体，导致封装在纳米孔中的药物分子释放到本体溶液中。荧光成像功能将允许非侵入性地跟踪体内的纳米颗粒。通过将药物递送与细胞成像能力相结合，这些纳米颗粒可以为生物医学应用提供有价值的多功能纳米平台。
A multifunctional system for intracellular drug delivery and simultaneous fluorescent imaging was constructed by using histidine-tagged, cyan fluorescent protein (CFP)-capped magnetic mesoporous silica nanoparticles (MMSNs). This protein-capped multifunctional nanostructure is highly biocompatible and does not affect cell viability or proliferation. The CFP acts not only as a capping agent, but also as a fluorescent imaging agent. The nanoassembly was activated by histidine-based replacement, leading to release of drug molecules encapsulated in the nanopores into the bulk solution. The fluorescent imaging functionality would allow noninvasive tracking of the nanoparticles in the body. By combining the drug delivery with cell-imaging capability, these nanoparticles may provide valuable multifunctional nanoplatforms for biomedical applications.