Dynamics and functional relevance of ammonia-oxidizing archaea in two agricultural soils
Dynamics and functional relevance of ammonia-oxidizing archaea in two agricultural soils
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DOI：
10.1111/j.1462-2920.2008.01783.x
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发表时间：
2009-02-01
影响因子：
5.1
通讯作者：
Schleper, Christa
中科院分区：
文献类型：
--
作者：
Schauss, Kristina;Focks, Andreas;Schleper, Christa
在许多情况下，地球化学循环的关键步骤是由一组以上的微生物完成的，但这种功能冗余对生态系统功能的重要性了解甚少。氨氧化古菌（AOA）和它们的细菌对应体（AOB）是解决这个问题的完美系统：尽管它们执行相同的转化步骤，但它们属于分离良好的系统发育类群。在猪粪中添加不同浓度的磺胺嘧啶（一种兽药中常用的抗生素），对AOB和AOA有不同程度的影响。添加肥料促进了两种土壤中AOA的生长，有趣的是，其中一种土壤中AOA的生长也得到了显著的促进。抗生素处理显著降低了有机肥对AOB的影响，而对AOA的影响程度较低。对氨氧化过程中AOA和AOB各自比例的模型计算表明，在SDZ的影响下，其中一种土壤中AOA的贡献很大，并进一步增加，因此AOA和AOB之间存在功能冗余。
Crucial steps in geochemical cycles are in many cases performed by more than one group of microorganisms, but the significance of this functional redundancy with respect to ecosystem functioning is poorly understood. Ammonia-oxidizing archaea (AOA) and their bacterial counterparts (AOB) are a perfect system to address this question: although performing the same transformation step, they belong to well-separated phylogenetic groups. Using pig manure amended with different concentrations of sulfadiazine (SDZ), an antibiotic that is frequently used in veterinary medicine, it was possible to affect AOB and AOA to different degrees. Addition of manure stimulated growth of AOB in both soils and, interestingly, also growth of AOA was considerably stimulated in one of the soils. The antibiotic treatments decreased the manure effect notably on AOB, whereas AOA were affected to a lower extent. Model calculations concerning the respective proportions of AOA and AOB in ammonia oxidation indicate a substantial contribution of AOA in one of the soils that further increased under the influence of SDZ, hence indicating functional redundancy between AOA and AOB.