Fibroblast growth factor-2-apatite composite layers on titanium screw to reduce pin tract infection rate.
Fibroblast growth factor-2-apatite composite layers on titanium screw to reduce pin tract infection rate.
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钛螺钉上成纤维细胞生长因子-2-磷灰石复合层可降低针道感染率。
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
N. Ochiai
中科院分区：
文献类型：
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作者：
H. Mutsuzaki;A. Ito;M. Sakane;Y. Sogo;A. Oyane;N. Ochiai
在阳极氧化钛螺钉上形成成纤维细胞生长因子-2（FGF-2）-磷灰石复合层，以提高骨螺钉界面强度，并通过增强外固定中的皮肤组织愈合来降低针道感染率。将添加有FGF-2的含钙溶液、含磷酸盐溶液和碳酸氢钠溶液以Ca/P摩尔比为2.0混合，以制备相对于磷酸钙过饱和的磷酸钙溶液。将螺钉单独浸入 37 ℃ 10 mL 磷酸钙溶液中 2 天。当过饱和磷酸钙溶液中的FGF-2浓度等于或低于10μg/mL时，在螺钉表面上形成掺有FGF-2的低结晶磷灰石层。固定在螺钉上的 FGF-2 的量为每个螺钉 2.3 至 2.4 杯。 NIH3T3 细胞增殖证明，固定化的 FGF-2 保留了生物活性。将复合层钛螺钉经皮植入家兔双侧胫骨近端干骺端，持续4周。在最佳FGF-2浓度下形成复合层的钛螺钉比没有复合层的螺钉表现出明显更高的骨螺钉界面强度和更低的钉道感染率：有复合层螺钉的拔出扭矩和感染率分别为0.230+/-0.073Nm和43.8%，无复合层螺钉的拔出扭矩和感染率分别为0.170+/-0.056Nm和93.8%。因此，具有FGF-2-磷灰石复合层的钛螺钉可有效提高骨螺钉界面强度和骨外固定中的抗感染能力。
Fibroblast growth factor-2 (FGF-2)-apatite composite layers were formed on anodically oxidized titanium screws to improve bone-screw interface strength and to reduce pin tract infection rate through enhanced skin tissue healing in external fixation. A calcium-containing solution supplemented with FGF-2, a phosphate-containing solution, and a sodium bicarbonate solution were mixed at a Ca/P molar ratio of 2.0 to prepare a calcium phosphate solution supersaturated with respect to calcium phosphates. Screws were individually immersed in 10 mL of the calcium phosphate solution at 37 degrees C for 2 days. Low-crystalline apatite layers incorporating FGF-2 were formed on the screw surface at FGF-2 concentrations in the supersaturated calcium phosphate solution equal to or lower than 10 mug/mL. The amounts of FGF-2 immobilized on the screws ranged from 2.3- to 2.4-mug per screw. The immobilized FGF-2 retained biological activity, as demonstrated by NIH3T3 cell proliferation. Titanium screws with the composite layer were percutaneously implanted into the bilateral proximal tibial metaphyses in rabbits for 4 weeks. The titanium screws with the composite layer formed at the optimum FGF-2 concentration showed a significantly higher bone-screw interface strength and a lower pin tract infection rate than those without the composite layer: the extraction torque and infection rates were respectively 0.230 +/- 0.073 Nm and 43.8% for the screws with the composite layer, and 0.170 +/- 0.056 Nm and 93.8% for those without the composite layer. Therefore, titanium screws with the FGF-2-apatite composite layer are useful for improving bone-screw interface strength and infection resistance in external skeletal fixation.
DOI：
10.1016/0169-6009(90)90015-8
发表时间：
1990-10-01
期刊：
BONE AND MINERAL
影响因子：
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作者：
BOSKEY, AL;MARESCA, M;VEIS, A
通讯作者：
VEIS, A