The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca.
The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca.
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DOI：
10.1111/febs.12778
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Ward JM
中科院分区：
文献类型：
--
作者：
Sayer C;Martinez-Torres RJ;Richter N;Isupov MN;Hailes HC;Littlechild JA;Ward JM
在过去十年中，转氨酶用于制药和精细化工中间体的生产引起了极大的关注。转氨酶是高效生产胺中间体的多功能生物催化剂，许多转氨酶具有(S)-对映体特异性。需要具有(R)特异性的转氨酶来扩大这些酶在生物催化中的应用。在这项工作中，我们从Eurotiomycetes Nectria haematococca中鉴定了一种真菌推定的(R)特异性转氨酶，克隆了该基因的合成版本，证明了几种底物(R)选择性脱胺，包括(R)-α-甲基苄胺，以及(R)-胺的生产，并确定了其晶体结构。全酶的晶体结构和与抑制剂加巴库林的复合物为工业上重要的一类(R)选择性胺：丙酮酸转氨酶的底物特异性和对映体选择性的结构基础提供了第一个详细的见解。全酶和gabaculine结合形式的Nectria TAm的原子坐标和结构因子分别作为4cmd和4cmf条目沉积在PDB中。•TAm和TAm通过x射线晶体学结合（查看相互作用）
During the last decade the use of transaminases for the production of pharmaceutical and fine chemical intermediates has attracted a great deal of attention. Transaminases are versatile biocatalysts for the efficient production of amine intermediates and many have (S)-enantiospecificity. Transaminases with (R)-specificity are needed to expand the applications of these enzymes in biocatalysis. In this work we have identified a fungal putative (R)-specific transaminase from the Eurotiomycetes Nectria haematococca, cloned a synthetic version of this gene, demonstrated (R)-selective deamination of several substrates including (R)-α-methylbenzylamine, as well as production of (R)-amines, and determined its crystal structure. The crystal structures of the holoenzyme and the complex with an inhibitor gabaculine offer the first detailed insight into the structural basis for substrate specificity and enantioselectivity of the industrially important class of (R)-selective amine : pyruvate transaminases. The atomic coordinates and structure factors for the Nectria TAm in holoenzyme and gabaculine-bound forms have been deposited in the PDB as entries 4cmd and 4cmf respectively. • TAm and TAm bind by x-ray crystallography (View interaction)
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影响因子：
5.8
作者：
Gouet, P;Courcelle, E;Métoz, F
通讯作者：
Métoz, F
DOI：
10.1111/j.1432-1033.1993.tb17953.x
发表时间：
1993-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
MEHTA, PK;HALE, TI;CHRISTEN, P
通讯作者：
CHRISTEN, P
影响因子：
2.9
作者：
Peisach, D;Chipman, DM;Ringe, D
通讯作者：
Ringe, D
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD
影响因子：
4.9
作者：
Hopwood, Jennifer;Truppo, Matthew D.;Lloyd, Richard C.
通讯作者：
Lloyd, Richard C.