Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels.
Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels.
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DOI：
10.1038/s41598-017-07255-z
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发表时间：
2017-07-31
影响因子：
4.6
通讯作者：
Dalby MJ
中科院分区：
文献类型：
--
作者：
Alakpa EV;Jayawarna V;Burgess KEV;West CC;Péault B;Ulijn RV;Dalby MJ
体外干细胞向软骨细胞的分化通常导致异质表型。这在经常检测到的X型胶原蛋白的过度表达中是明显的，在透明软骨结构中，X型胶原蛋白不是中间区的特征，而是深区骨化组织的特征。因此，在再生医学中非常需要将体外发育的软骨与体内特征更好地匹配的方法。本研究比较了从人脂肪组织中获得的周细胞之间的表型特征，所述周细胞使用二苯丙氨酸/丝氨酸（F2/S）肽水凝胶与更广泛使用的用于软骨形成的化学诱导方法分化。当周细胞在没有诱导培养基的情况下在水凝胶中进行软骨形成时，注意到II型胶原蛋白的水平显著较高。还存在胶原蛋白相对于聚集蛋白聚糖产生的平衡表达，当细胞用诱导培养基培养时，该特征偏向于胶原蛋白产生。最后，每个系统的代谢谱显示两种分化方法之间有相当大的重叠，但可以确定潜在引起其所得表型的细微差异。该研究强调了细胞微环境中的材料和化学变化如何对所得组织类型产生广泛影响。
Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.
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影响因子：
41.2
作者：
通讯作者：
--
影响因子：
9.7
作者：
Jayawarna, Vineetha;Richardson, Stephen M.;Ulijn, Rein V.
通讯作者：
Ulijn, Rein V.
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM