Changes in macrophage function modulated by the lipid environment.
Changes in macrophage function modulated by the lipid environment.
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DOI：
10.1177/1753425916633886
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发表时间：
2016-04
期刊：
影响因子：
3.2
通讯作者：
De Maio A
中科院分区：
文献类型：
--
作者：
Williams MR;Cauvi DM;Rivera I;Hawisher D;De Maio A
巨噬细胞（macrophages，M β）在防御病原体、协调损伤期间的炎症反应和维持组织稳态中起关键作用。在这些过程中，巨噬细胞会遇到各种可能改变其基因表达模式的环境条件，从而调节其功能。在这项研究中，我们发现，小鼠M β细胞显示两个不同的亚群，其特征在于在非刺激条件下的形态，表面标志物的表达和吞噬能力的差异。这两个亚群可以通过培养条件的变化来概括。因此，在血清存在下悬浮生长的M β是高度吞噬的，而血清的减少导致快速附着和减少的吞噬活性。这些亚群之间吞噬功能的差异与FcγR的表达水平相关。这两个细胞亚群对LPS的反应和表面标志物的表达也不同，包括CD 14，CD 86，清道夫受体A1，TLR 4和低密度脂蛋白受体。此外，我们发现培养基中的脂质/胆固醇含量介导了这两种细胞亚群之间的差异。因此，我们描述了一种调节M β功能的机制，该机制取决于其周围微环境中的脂质暴露。
Macrophages (Mϕs) play a critical role in the defense against pathogens, orchestrating the inflammatory response during injury and maintaining tissue homeostasis. During these processes, macrophages encounter a variety of environmental conditions that are likely to change their gene expression pattern, which modulates their function. In this study, we found that murine Mϕs displayed two different subpopulations characterized by differences in morphologies, expression of surface markers and phagocytic capacity under non-stimulated conditions. These two subpopulations could be recapitulated by changes in the culture conditions. Thus, Mϕs grown in suspension in the presence of serum were highly phagocytic, whereas subtraction of serum resulted in rapid attachment and reduced phagocytic activity. The difference in phagocytosis between these subpopulations was correlated with the expression levels of FcγR. These two cell subpopulations also differed in their responses to LPS and the expression of surface markers, including CD14, CD86, scavenger receptor A1, TLR4 and low-density lipoprotein receptor. Moreover, we found that the lipid/cholesterol content in the culture medium mediated the differences between these two cell subpopulations. Thus, we described a mechanism that modulates Mϕ function depending on the exposure to lipids within their surrounding microenvironment.
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影响因子：
8.7
作者：
Beutler B
通讯作者：
Beutler B
DOI：
10.1016/j.jss.2010.08.005
发表时间：
2012-03
期刊：
The Journal of surgical research
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
20.3
作者：
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通讯作者：
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