Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent
Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent
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DOI：
10.1016/j.cca.2010.09.040
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发表时间：
2011-01-14
影响因子：
5
通讯作者：
Nakajima, Katsuyuki
中科院分区：
文献类型：
--
作者：
Nakano, Takamitsu;Tokita, Yoshiharu;Nakajima, Katsuyuki
背景：血清残余样脂蛋白颗粒（RLP）浓度已被胆固醇测定作为RLP- c用于临床诊断。然而，RLP中TG的测定方法以及RLP-TG/总TG的比值尚未得到很好的确定。方法：采用高灵敏甘油三酯测定试剂TG- ex进行RLP-TG测定，并与之前使用的TG测定试剂LTGII进行比较。健康体检人群、心血管疾病患者、糖尿病患者和口腔脂肪负荷患者的血清被用于评估新的RLP-TG检测。同时测定血清TC、TG、HDL-C、LDL-C和RLP-C浓度。结果：TG-EX对新RLP-TG的检出限为2.0 mg/dl。运行内不精密度（n = 10） CV = 3.0% (RLP-TG: 4.1 mg +/- 0.7 mg/dl), CV = 1.4% (RLP-TG: 42.0 +/- 0.6 mg/dl), CV = 0.5% （RLP-TG: 100.6 +/- 0.6 mg/dl）。在禁食的日本人群中，RLP-TG的临界值（75%百分位数）为男性13.1 mg/dl，女性9.9 mg/dl。用于代谢综合征、心血管疾病和糖尿病患者。RLP-TG水平明显高于正常对照组。口服脂肪负荷后，RLP-TG水平显著升高，RLP-TG/总TG的比值比禁食状态增加了3倍。口服脂肪负荷后，大约80%的TG增加是来自残余脂蛋白的TG。结论：日本空腹人群血浆RLP-TG的正常范围首先用高灵敏度TG测定试剂测定。RLP-TG在代谢综合征、心血管疾病患者中较高。等，是比RLP-C更好的测定餐后残余脂蛋白和总TG的标记物，RLP-TG/总TG比值。(C) 2010 Elsevier B.V.版权所有
Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established.Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases.Results: The detection limit of new RLP-TG using TG-EX was 2.0 mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1 mg +/- 0.7 mg/dl), CV = 1.4% (RLP-TG: 42.0 +/- 0.6 mg/dl) and CV = 0.5% (RLP-TG: 100.6 +/- 0.6 mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1 mg/dl in men and 9.9 mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes. RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased>3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins.Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease. etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio. (C) 2010 Elsevier B.V. All rights reserved.