Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins
Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins
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DOI：
10.1016/s0092-8674(00)80425-0
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发表时间：
1997-10-31
期刊：
影响因子：
64.5
通讯作者：
OConnor, MB
中科院分区：
文献类型：
--
作者：
Marques, G;Musacchio, M;OConnor, MB
在果蝇早期胚胎发生过程中，几个合子基因产物作用于形态原DPP的转录后活性梯度的建立。在这些分子中，Tolloid，一种被认为与BMP-1相关的金属蛋白酶，增强DPP功能，而SOG，一种爪蟾组织者Chordin的同源物，抑制DPP功能。通过上位性实验和爪蟾次级轴诱导实验，我们发现TLD可以消除SOG/CHD对DPP/ bmp型配体的抑制作用。在瞬时转染实验中，我们证明TLD可以切割SOG，并且这种切割是由DPP刺激的。我们提出胚胎DPP活性梯度的形成涉及SOG抑制DPP和TLD处理SOG以从抑制复合物中释放DPP的相反作用。
During early Drosophila embryogenesis, several zygotic gene products act to establish a posttranscriptional activity gradient of the morphogen DPP. Among these molecules, Tolloid, a putative metalloprotease related to BMP-1, enhances DPP function, while SOG, an ortholog of the Xenopus organizer Chordin, inhibits DPP function. Using epistasis tests and a Xenopus secondary axis induction assay, we show that TLD negates the inhibitory effects of SOG/CHD on DPP/BMP-type ligands. In transient transfection assays, we demonstrate that TLD cleaves SOG and that cleavage is stimulated by DPP. We propose that formation of the embryonic DPP activity gradient involves the opposing effects of SOG inhibiting DPP and TLD processing SOG to release DPP from the inhibitory complex.