Cooperative Responses of Multiple Kinesins to Variable and Constant Loads
Cooperative Responses of Multiple Kinesins to Variable and Constant Loads
复制标题
DOI：
10.1074/jbc.m111.296582
复制
发表时间：
2012-01-27
影响因子：
4.8
通讯作者：
Diehl, Michael R.
中科院分区：
文献类型：
--
作者：
Jamison, D. Kenneth;Driver, Jonathan W.;Diehl, Michael R.
微管依赖性运输最常见的驱动蛋白和动力蛋白的集合，在一个协调的方式或拮抗功能。一些证据表明，货物运输可能不会受到明显的影响，由多个驱动蛋白的联合行动。然而，在以前的光学捕获实验中，施加在货物上的力将根据一些局部环境因素在细胞中在空间和时间上变化，这些条件的影响在很大程度上被忽视了。在这里，我们的特点是动态的结构定义的复合物包含多个驱动蛋白的光学力钳的控制负载下。虽然证明有通用的动力障碍，限制多个驱动蛋白的合作能力，生产力，施加负载的空间和时间属性被发现在多个电机系统的集体动力学中发挥重要作用。我们建议这种依赖性的影响细胞内的运输过程，特别是双向运输。
Microtubule-dependent transport is most often driven by collections of kinesins and dyneins that function in either a concerted fashion or antagonistically. Several lines of evidence suggest that cargo transport may not be influenced appreciably by the combined action of multiple kinesins. Yet, as in previous optical trapping experiments, the forces imposed on cargos will vary spatially and temporally in cells depending on a number of local environmental factors, and the influence of these conditions has been largely overlooked. Here, we characterize the dynamics of structurally defined complexes containing multiple kinesins under the controlled loads of an optical force clamp. While demonstrating that there are generic kinetic barriers that restrict the ability of multiple kinesins to cooperate productively, the spatial and temporal properties of applied loads is found to play an important role in the collective dynamics of multiple motor systems. We propose this dependence has implications for intracellular transport processes, especially for bidirectional transport.