Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.
Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.
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DOI：
10.1371/journal.pone.0090181
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Li L;Zhang Z;Wang C;Miao L;Zhang J;Wang J;Jiao B;Zhao S
为了寻找喉癌诊断和检测的候选生物标志物，并探讨喉癌发生的可能机制，采用二维强阳离子交换/反相纳米液相色谱/质谱分析技术，对喉癌组织和癌旁正常组织中差异表达的蛋白质进行鉴定。结果，鉴定出281个具有显著差异表达的蛋白，并选择性地验证了具有不同亚细胞定位的4个差异蛋白，Profilin-1（PFN 1）、Nucleolin（NCL）、胞质非特异性二肽酶（CNDP 2）和Mimecan（OGN）。采用半定量RT-PCR和Western blotting方法检测4种蛋白在喉癌组织中的表达，结果验证了蛋白质组学鉴定的差异表达蛋白。此外，我们敲低PFN 1在永生化的人喉鳞状细胞系Hep-2细胞，然后这些转染细胞的增殖和转移的测量。结果表明，PFN 1基因沉默抑制了Hep-2细胞的增殖，并影响了Hep-2细胞的迁移能力，为进一步研究PFN 1在喉癌中的作用机制提供了新的思路。本研究首次发现PFN 1、NCL、CNDP 2和OGN是喉癌诊断和治疗的潜在生物标志物，PFN 1与喉癌转移有关。
To discover candidate biomarkers for diagnosis and detection of human laryngeal carcinoma and explore possible mechanisms of this cancer carcinogenesis, two-dimensional strong cation-exchange/reversed-phase nano-scale liquid chromatography/mass spectrometry analysis was used to identify differentially expressed proteins between the laryngeal carcinoma tissue and the adjacent normal tissue. As a result, 281 proteins with significant difference in expression were identified, and four differential proteins, Profilin-1 (PFN1), Nucleolin (NCL), Cytosolic non-specific dipeptidase (CNDP2) and Mimecan (OGN) with different subcellular localization were selectively validated. Semiquantitative RT-PCR and Western blotting were performed to detect the expression of the four proteins employing a large collection of human laryngeal carcinoma tissues, and the results validated the differentially expressed proteins identified by the proteomics. Furthermore, we knocked down PFN1 in immortalized human laryngeal squamous cell line Hep-2 cells and then the proliferation and metastasis of these transfected cells were measured. The results showed that PFN1 silencing inhibited the proliferation and affected the migration ability of Hep-2 cells, providing some new insights into the pathogenesis of PFN1 in laryngeal carcinoma. Altogether, our present data first time show that PFN1, NCL, CNDP2 and OGN are novel potential biomarkers for diagnosis and therapeutic targets for laryngeal carcinoma, and PFN1 is involved in the metastasis of laryngeal carcinoma.
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影响因子：
8
作者：
Orr, B.;Riddick, A. C. P.;Stewart, G. D.;Anderson, R. A.;Franco, O. E.;Hayward, S. W.;Thomson, A. A.
通讯作者：
Thomson, A. A.
正常喉部病变至癌前病变及喉鳞状细胞癌蛋白质组转变的初步研究
影响因子：
1.4
作者：
Cheng, Lei;Zhou, Liang;Liu, Yinkun
通讯作者：
Liu, Yinkun
影响因子：
--
作者：
Liu, Chibo;Pan, Chunqin;Yong, Liang
通讯作者：
Yong, Liang
影响因子：
11.5
作者：
Peng, Lan;Liang, John;Wang, Huamin
通讯作者：
Wang, Huamin
影响因子：
50.3
作者：
Mouneimne G;Hansen SD;Selfors LM;Petrak L;Hickey MM;Gallegos LL;Simpson KJ;Lim J;Gertler FB;Hartwig JH;Mullins RD;Brugge JS
通讯作者：
Brugge JS