INTERLEUKIN-1-BETA INDUCES CYTOSOLIC PLA2 IN PARALLEL WITH PROSTAGLANDIN E(2) IN RHEUMATOID SYNOVIAL FIBROBLASTS
INTERLEUKIN-1-BETA INDUCES CYTOSOLIC PLA2 IN PARALLEL WITH PROSTAGLANDIN E(2) IN RHEUMATOID SYNOVIAL FIBROBLASTS
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DOI：
10.1007/bf01972703
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
MORGAN, DW
中科院分区：
文献类型：
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作者：
HULKOWER, KI;COFFEY, JW;MORGAN, DW
我们研究了白细胞介素-1 β（IL-1 β）处理的类风湿性滑膜成纤维细胞（RSF）中酶活性增加与高分子量（100 kDa）蛋白质，胞浆PLA 2（cPLA 2）之间的时间关系。这两种反应都根据与这些细胞产生PGE 2相关的类似时程增加。相反，14 kDa分泌型PLA 2（sPLA 2），也是由RSF产生的，不受IL-1 β处理的影响。这些发现支持了cPLA 2活性的增强，由cPLA 2蛋白而不是sPLA 2的诱导引起，与IL-1 β处理的RSF中PGE 2产生的增加在时间上相关。
We investigated the temporal relationship between the increase in enzymatic activity and protein of a high molecular weight (100 kDa), cytosolic PLA2 (cPLA2) in interleukin-1beta (IL-1beta)-treated rheumatoid synovial fibroblasts (RSF). Both of these responses increased according to a similar time-course which correlates with PGE2 production by these cells. In contrast, 14 kDa, secreted PLA2 (sPLA2), which was also produced by RSF, was not affected by IL-1beta treatment. These findings support that an augmentation of cPLA2 activity, caused by an induction of cPLA2 protein, rather than sPLA2, is temporally associated with increased PGE2 production in IL-1beta-treated RSF.