Classification of DLC films for cell proliferation based on optical constants
Classification of DLC films for cell proliferation based on optical constants
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DOI：
10.1016/j.diamond.2021.108266
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发表时间：
2021-01-22
影响因子：
4.1
通讯作者：
Hirakuri, Kenji
中科院分区：
文献类型：
--
作者：
Kanasugi, Kazuya;Ohgoe, Yasuharu;Hirakuri, Kenji
采用物理气相沉积和化学气相沉积两种方法，在316L不锈钢表面制备了9种类金刚石（DLC）薄膜。用光谱椭偏仪测定了DLC薄膜的折射率和消光系数。DLC样品分为a-C型、a-C:H型、类聚合物型和类石墨型4种类型。此外，用x射线光电子能谱和细胞培养分析了DLC膜的表面功能。DLC膜具有较高的消光系数和C-O键比，细胞增殖水平较高。消光系数与细胞在DLC膜上的增殖有关，并与膜的结构和沉积方法有关。利用光学特性，如消光系数，可以区分不同类型的DLC膜，细胞可以在其上生长作为生物界面。
Nine types of diamond-like carbon (DLC) films were prepared on 316L stainless steel by physical vapor deposition and chemical vapor deposition under different plasma processing conditions. The refractive index and extinction coefficient of the DLC films were evaluated with a spectroscopic ellipsometer. The DLC samples were classified into 4 types of films: a-C, a-C:H, polymer-like, and graphite-like types. In addition, the surface functions of the DLC films were analyzed by X-ray photoelectron spectroscopy and cell culture. DLC films on which high levels of cell proliferation were observed had relative high extinction coefficients and C-O bond ratios. The extinction coefficient was related to cell proliferation on the DLC films and depended on the film structure and deposition methods. Using optical properties, such as the extinction coefficient, it is possible to distinguish various types of DLC films on which cells can be grown as a biointerface.