The expression of B-cadherin during embryonic chick development.
The expression of B-cadherin during embryonic chick development.
复制标题
B-钙粘蛋白在鸡胚发育过程中的表达。
DOI：
10.1006/dbio.1994.1013
复制
发表时间：
1994
影响因子：
2.7
通讯作者：
Reichardt,LF
中科院分区：
文献类型：
--
作者：
Murphy-Erdosh,C;Napolitano,EW;Reichardt,LF
钙粘蛋白是一类钙依赖性细胞粘附分子，在发育过程中参与分化组织的分离。每一种钙粘蛋白都有独特的时空表达模式。正如其他钙粘蛋白所观察到的，当b -钙粘蛋白在小鼠L929成纤维细胞中表达时，赋予它们钙依赖性的细胞聚集活性。分离了一种B-cadherin单克隆抗体，用于测定B-cadherin在发育中的鸡胚中的表达规律。抗体染色和原位杂交显示，B-cadherin蛋白和mRNA存在于来自三个初级生殖层的多种上皮细胞中，它们的表达在分化过程中受到显著调节。在某些情况下，组织内b -钙粘蛋白的区域分布反映了组织的不同功能区域。染色模式与抗lcam抗体相似，但不同。虽然B-cadherin和LCAM共表达的例子很多，但也存在明显的区域差异。
Cadherins compose a family of calcium-dependent cell adhesion molecules that are involved in the segregation of differentiating tissues during development. Each cadherin has a unique spatial and temporal pattern of expression. As has been observed for other cadherins, B-cadherin, when expressed in mouse L929 fibroblasts, confers upon them a calcium-dependent cell aggregation activity. A monoclonal antibody to B-cadherin was isolated and used to determine the pattern of expression of B-cadherin in the developing chick embryo. Antibody staining andin situhybridization reveal that B-cadherin protein and mRNA are found in diverse epithelia derived from each of the three primary germ layers, where their expression is strikingly regulated during differentiation. In some instances, the regional distribution of B-cadherin within a tissue reflects the distinct functional regions of the tissue. Patterns of staining are similar to, but distinct from, those seen with anti-LCAM antibodies. Although there are many examples where B-cadherin and LCAM are coexpressed, there are also distinctive regional differences.