Circulating cell-free DNA, peripheral lymphocyte subsets alterations and neutrophil lymphocyte ratio in assessment of COVID-19 severity.
Circulating cell-free DNA, peripheral lymphocyte subsets alterations and neutrophil lymphocyte ratio in assessment of COVID-19 severity.
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DOI：
10.1177/1753425921995577
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发表时间：
2021-04
期刊：
影响因子：
3.2
通讯作者：
Abdelmaksoud MF
中科院分区：
文献类型：
--
作者：
Hammad R;Eldosoky MAER;Fouad SH;Elgendy A;Tawfeik AM;Alboraie M;Abdelmaksoud MF
细胞破坏导致无细胞DNA(CfDNA)的胞浆积聚。循环中淋巴细胞的动态变化是新冠肺炎的特点。我们旨在研究cfDNA水平是否可以用于新冠肺炎患者的分层，以及cfDNA水平是否与淋巴细胞亚群和中性粒细胞/淋巴细胞比率的变化有关。本次横断面比较研究纳入SARS冠状病毒2型阳性患者。患者被细分为重症组和非重症组。用实时荧光定量聚合酶链式反应测定血浆cfDNA浓度。用流式细胞仪检测淋巴细胞亚群。重症患者cfDNA水平明显高于非重症患者。CFDNA与NLR呈正相关，与T细胞百分比呈负相关。CfDNA与铁蛋白、C反应蛋白呈正相关。ROC曲线的输出结果显示，cfDNA在≥为17.31 ng/µL，AUC0.96时的敏感性为93%，特异性为73%。综上所述，cfDNA的过度释放可作为新冠肺炎病情严重程度的敏感预测指标。CfDNA上调与NLR上调和T细胞百分率下调有关。CfDNA水平可用于新冠肺炎患者的分层和个性化监测策略。
Cell destruction results in plasma accumulation of cell-free DNA (cfDNA). Dynamic changes in circulating lymphocytes are features of COVID-19. We aimed to investigate if cfDNA level can serve in stratification of COVID-19 patients, and if cfDNA level is associated with alterations in lymphocyte subsets and neutrophil-to-lymphocyte ratio (NLR). This cross-sectional comparative study enrolled 64 SARS-CoV-2-positive patients. Patients were subdivided to severe and non-severe groups. Plasma cfDNA concentration was determined by real-time quantitative PCR. Lymphocyte subsets were assessed by flow cytometry. There was significant increase in cfDNA among severe cases when compared with non-severe cases. cfDNA showed positive correlation with NLR and inverse correlation with T cell percentage. cfDNA positively correlated with ferritin and C-reactive protein. The output data of performed ROC curves to differentiate severe from non-severe cases revealed that cfDNA at cut-off ≥17.31 ng/µl and AUC of 0.96 yielded (93%) sensitivity and (73%) specificity. In summary, excessive release of cfDNA can serve as sensitive COVID-19 severity predictor. There is an association between cfDNA up-regulation and NLR up-regulation and T cell percentage down-regulation. cfDNA level can be used in stratification and personalized monitoring strategies in COVID-19 patients.
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影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
28.2
作者：
Liu, Yuwei;Du, Xuebei;Zhao, Yan
通讯作者：
Zhao, Yan
影响因子：
11.8
作者：
Qin, Chuan;Zhou, Luoqi;Tian, Dai-Shi
通讯作者：
Tian, Dai-Shi
影响因子：
8.4
作者：
Deng, Zhifeng;Zhang, Minli;Xu, Yu
通讯作者：
Xu, Yu
DOI：
10.1007/s13167-020-00226-x
发表时间：
2020-12
期刊：
The EPMA journal
影响因子：
--
作者：
Crigna AT;Samec M;Koklesova L;Liskova A;Giordano FA;Kubatka P;Golubnitschaja O
通讯作者：
Golubnitschaja O