Actions of endothelin on isolated corpus cavernosum from rabbit and man.
Actions of endothelin on isolated corpus cavernosum from rabbit and man.
复制标题
内皮素对兔和人离体海绵体的作用。
DOI：
--
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
H. Hedlund
中科院分区：
文献类型：
--
作者：
F. Holmquist;K. Andersson;H. Hedlund
本实验观察了血管内皮细胞产生的缩血管活性多肽--内皮素对兔和人海绵体的作用。兔和人的制剂都能被内皮素以浓度依赖的方式有效地收缩。收缩发展缓慢，即使频繁洗涤也不能逆转，而且只有部分被钙通道阻滞剂尼莫地平抑制。即使在含有螯合剂EGTA的无钙培养液中，也有一个小的收缩成分持续存在。在兔CC中，无钙介质中的收缩不受尼莫地平、钙通道激动剂Bay K 86(44)或耗尽细胞内对去甲肾上腺素(NA)和咖啡因敏感的钙离子储存库的影响，但几乎被蛋白激酶C抑制剂H7所阻断。在兔和人中，卡巴胆碱和血管活性肠多肽浓度依赖地松弛由内皮素收缩的制剂。阿托品可拮抗卡巴胆碱的松弛作用。内皮素可浓度依赖地增强外源性NA引起的兔心收缩。这种增强作用在低浓度NA时更为明显。这项研究表明，内皮素可以有效地收缩分离的阴茎勃起组织。这种收缩似乎主要是通过钙离子通过细胞膜的内流来调节的，这部分是通过电压操纵的钙通道以外的另一种途径发生的。然而，不能排除其他机制的参与。结果提示，内皮素在阴茎勃起机制中可能起一定作用。
The effects of endothelin, a vasoconstrictor peptide produced by vascular endothelial cells, were investigated in isolated rabbit and human corpus cavernosum (CC). Preparations from both rabbit and man were potently contracted by endothelin in a concentration-dependent manner. The contractions developed slowly, could not be reversed despite frequent washings, and were only partly inhibited by the Ca2+ channel blocker nimodipine. Even in Ca2(+)-free medium containing the chelator EGTA a small contractile component persisted. In rabbit CC, the contractions in Ca2(+)-free medium were not affected by nimodipine, the Ca2(-)-channel agonist BAY K 86(44), or by depletion of intracellular Ca2(+) stores sensitive to noradrenaline (NA) and caffeine, but were almost abolished by the protein kinase C inhibitor H7. In both rabbit and man, carbachol and vasoactive intestinal polypeptide concentration-dependently relaxed preparations contracted by endothelin. The relaxations induced by carbachol were antagonized by atropine. Endothelin enhanced concentration-dependently the contractions induced by exogenously applied NA in rabbit CC. The enhancement was more pronounced at low concentrations of NA. This study shows that endothelin potently contracts isolated penile erectile tissue. The contraction seems to be mediated mainly by influx of Ca2+ through the cell membrane, which partly occurs through a pathway other than voltage-operated calcium channels. However, involvement of other mechanisms cannot be excluded. The results suggest that endothelin can play a role in penile erectile mechanisms.
内皮素作用：蛋白激酶 C 抑制剂的抑制和磷酸肌醇的参与。
DOI：
10.1016/s0006-291x(89)80193-7
发表时间：
1989
影响因子：
3.1
作者：
Sugiura,M;Inagami,T;Hare,GM;Johns,JA
通讯作者：
Johns,JA