MiRNA-target pairs regulate adventitious rooting in Populus: a functional role for miR167a and its target Auxin response factor 8
MiRNA-target pairs regulate adventitious rooting in Populus: a functional role for miR167a and its target Auxin response factor 8
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miRNA-靶标对调节杨属不定根：miR167a 及其靶标生长素反应因子 8 的功能作用
DOI：
10.1093/treephys/tpz085
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发表时间：
2019-11-01
期刊：
影响因子：
4
通讯作者：
Xu, Meng
中科院分区：
文献类型：
--
作者：
Cai, Heng;Yang, Chunxia;Xu, Meng
植物从其非根组织形成根的能力在快速适应变化的环境期间在生态上是有利的。虽然这种生物现象已被广泛用于许多经济上重要的农艺和树种的扦插，但其遗传和发育机制一直知之甚少。本研究对白杨嫩枝扦插生根过程中的小RNA、降解组和转录组进行了关联分析，共检测到373对miRNA-靶对。其中，72个显著差异表达的靶标被筛选为可能与植物激素信号转导一起调节不定根（AR）发育。对白杨miR 167 a及其靶PeARF 6 s和PeARF 8 s介导植物生长和激素信号转导的能力进行功能验证。miR 167 a的过表达抑制了靶基因转录本的表达并促进了白杨侧根的发育，而PeARF8.1（mut）的过表达增加了侧根的数量并轻微抑制了侧根的发育。这些结果表明，miR 167 a-PeARF 8.1模块在调控白杨AR和LR发育中发挥着重要作用，提高了白杨对更复杂环境的适应能力。
The ability of a plant to form roots from its non-root tissues is ecologically advantageous during rapid adaptation to a changing environment. Although this biological phenomenon has been widely utilized for cuttings in many economically important agronomic and tree species, its genetic and developmental mechanisms have been poorly understood. In this study, we conducted an association analysis of small RNAs, the degradome and the transcriptome of adventitious rooting in poplar softwood cuttings, which revealed that 373 miRNA-target pairs were detected. Of these, 72 significantly differentially expressed targets were screened as likely to modulate adventitious root (AR) development, in conjunction with plant hormone signal transduction. Poplar miR167a and its targets PeARF6s and PeARF8s were subjected to functional verification of their ability to mediate plant growth and hormone signal transduction. Overexpression of miR167a inhibited target transcripts and improved lateral root (LR) development in poplar, while overexpressing PeARF8.1(mut) increased AR numbers and slightly inhibited LR development. Taken together, these results suggest that miR167a-PeARF8.1 modules play crucial roles in regulating AR and LR development in poplar and improve the adaptation of poplar to more complex environments.