Bioassays guided isolation of compounds from Chaetomium globosum.
Bioassays guided isolation of compounds from Chaetomium globosum.
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DOI：
10.1016/j.mycmed.2013.10.005
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发表时间：
2014-06-01
影响因子：
3.6
通讯作者：
El-Feky, A M M
中科院分区：
文献类型：
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作者：
Awad, N E;Kassem, H A;El-Feky, A M M
本研究的目的是评价不同的生物活性的真菌球毛壳（科毛壳）。通过测试其抗菌、抗氧化和抗癌作用进行评价。从黄瓜根际土壤中分离到一株球形假丝酵母，在复种试验中对茄病镰刀菌、立枯丝核菌和白绢病菌的菌丝生长均有抑制作用。结果表明，球毛藻液体培养物的石油醚和乙酸乙酯提取物具有较强的体外抗氧化活性。球形梭菌对枯草芽孢杆菌、大肠杆菌和荧光假单胞菌具有较强的抗菌活性。它还记录了对白色念珠菌、茄病镰刀菌、尖孢镰刀菌、茄病镰刀菌和终极腐霉的显著抗真菌活性。对人肝癌细胞株HepG2有明显的细胞毒作用。石油醚提取物的不皂化物和皂化物显示存在烃、甾醇和脂肪酸。乙酸乙酯提取物中含有Prenisatin、大黄酚、chrysazin、毛绿菌素A和B。分离的次级代谢产物证明了显着的抗氧化剂和抑菌活性的B.枯草杆菌，大肠杆菌和R.茄。综上所述，该真菌具有不同的生物活性。在农业和医药领域的应用还有待进一步研究。
The aim of the present study was to evaluate different biological activities of the fungus Chaetomium globosum (family Chaetomiaceae). The evaluation was done through testing its antimicrobial, antioxidant and anticancer effects. C.globosum was isolated from the Cucumber soil (rhizosphere) and caused inhibition of the mycelial growth of Fusarium solani, Rhizoctonia solani and Sclerotium rolfsii in the biculture test. Petroleum ether and ethyl acetate extracts of the liquid culture of C.globosum showed potent in vitro antioxidant activity. C.globosum proved potent antibacterial activity against Bacillus subtilis, Escherichia coli and Pseudomonas fluorescens. It also recorded significant antifungal activity against Candida albicans, F.solani, Fusarium oxysporum, R.solani and Pythium ultimum. It exerted cytotoxic effect on human hepatocellular carcinoma cell line (HepG2). Unsaponifiable and saponifiable matters of the petroleum ether extract showed the presence of hydrocarbons, sterols and fatty acids. The ethyl acetate extract showed the presence of prenisatin, chrysophanol, chrysazin, chaetoviridin A and B. The isolated secondary metabolites proved significant antioxidant and antimicrobial activity on B.subtilis, E.coli and R.solani. In conclusion, this fungus showed different biological activities. Further studies must be done to apply its use in the agricultural and medicinal field.