Production and purification of Clostridium perfringens alpha-toxin using a protein-hyperproducing strain, Bacillus brevis 47.
Production and purification of Clostridium perfringens alpha-toxin using a protein-hyperproducing strain, Bacillus brevis 47.
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使用蛋白质高产菌株短芽孢杆菌 47 生产和纯化产气荚膜梭菌 α 毒素。
DOI：
10.1111/j.1574-6968.1996.tb08584.x
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发表时间：
1996
影响因子：
2.1
通讯作者：
J. Sakùrai
中科院分区：
文献类型：
--
作者：
M. Nagahama;K. Michiue;J. Sakùrai
将产气荚膜梭菌α-毒素基因克隆到含有外细胞壁蛋白基因多个启动子和信号肽编码区的表达-分泌载体中，在蛋白高产菌株短杆菌47中表达。携带该基因的短小芽孢杆菌47转化子产生的α-毒素约为携带该毒素基因的枯草杆菌转化子的10倍。短小芽孢杆菌47转化子分泌的毒素具有与野生型α-毒素相同的生物学活性和N端氨基酸序列。
Clostridium perfringens alpha-toxin was produced in a protein-hyperproducing strain, Bacillus brevis 47, by cloning the gene into the constructed expression-secretion vector which has the multiple promoters and the signal peptide coding region of an outer cell wall protein gene. The amount of alpha-toxin produced by the B. brevis 47 transformant carrying the gene was approximately 10 times greater than that produced by a B. subtilis transformant carrying the toxin gene. Biological activities and the N-terminal amino acid sequence of the toxin secreted by the B. brevis 47 transformant were identical to those of wild-type alpha-toxin.