Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells
Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells
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DOI：
10.1002/cbin.10692
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发表时间：
2016-12
影响因子：
3.9
通讯作者：
Y. Hosokawa;I. Hosokawa;Satoru Shindo;Y. Ohta;K. Ozaki;T. Matsuo
中科院分区：
文献类型：
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作者：
Y. Hosokawa;I. Hosokawa;Satoru Shindo;Y. Ohta;K. Ozaki;T. Matsuo
在琉璃苣家族的成员Alkanna tinctoria中发现的Alkannin被用作食用色素。最近有报道称紫草素具有一定的生物学功能，如抗微生物和抗氧化作用。已知CC趋化因子受体（CCR）5阳性白细胞有助于牙周病变中的牙槽骨吸收。本研究的目的是检查紫草素是否抑制人牙周膜细胞（HPDLC）中CC趋化因子配体（CCL）3和CCL 5（CCR 5配体）的产生。白细胞介素（IL）-1 β诱导HPDLC产生CCL 3和CCL 5。紫草素以剂量依赖性方式抑制HPDLC中IL-1β介导的CCL 3和CCL 5产生。此外，我们发现紫草素抑制IL-1β刺激的HPDLC中kappa B-α降解的抑制剂。此外，核因子（NF）-κB抑制剂显著抑制IL-1β刺激的HPDLC中CCL 3和CCL 5的产生。这些结果表明，紫草素通过减弱NF-κB信号通路抑制IL-1β刺激的HPDLC中的CCR 5配体产生。
Alkannin, which is found in Alkanna tinctoria, a member of the borage family, is used as a food coloring. Alkannin has recently been reported to have certain biological functions, such as anti‐microbial and anti‐oxidant effects. It is known that CC chemokine receptor (CCR) 5‐positive leukocytes contribute to alveolar bone resorption in periodontal lesions. The aim of this study was to examine whether alkannin inhibits the production of CC chemokine ligand (CCL) 3 and CCL5, which are CCR5 ligands, in human periodontal ligament cells (HPDLC). Interleukin (IL)‐1β induced CCL3 and CCL5 production in HPDLC. Alkannin inhibited IL‐1β‐mediated CCL3 and CCL5 production in HPDLC in a dose‐dependent manner. Moreover, we revealed that alkannin suppressed inhibitor of kappa B‐α degradation in IL‐1β‐stimulated HPDLC. In addition, a nuclear factor (NF)‐κB inhibitor significantly inhibited CCL3 and CCL5 production in IL‐1β‐stimulated HPDLC. These results demonstrate that alkannin inhibits CCR5 ligand production in IL‐1β‐stimulated HPDLC by attenuating the NF‐κB signaling pathway.