polished ricemediates ecdysone‐dependent control ofDrosophilaembryonic organogenesis
polished ricemediates ecdysone‐dependent control ofDrosophilaembryonic organogenesis
复制标题
精米介导果蝇胚胎器官发生的蜕皮激素依赖性控制
DOI：
10.1111/gtc.12841
复制
发表时间：
2021
期刊：
影响因子：
2.1
通讯作者：
Kageyama Yuji
中科院分区：
文献类型：
--
作者：
Taira Yuki;Wada Housei;Hayashi Shigeo;Kageyama Yuji
在许多动物中，发育阶段的进展在时间上由类固醇激素控制。在果蝇中，蜕皮激素滴度的水平振荡和发育阶段的转变，如幼虫蜕皮和变态，诱导在每个蜕皮激素峰。蜕皮激素滴度也在胚胎发生中期达到峰值，胚胎蜕皮激素是几个器官形态发生所必需的，尽管依赖于蜕皮激素信号传导的胚胎器官发生的调节机制仍然是悬而未决的问题。在这项研究中，我们发现，胚胎蜕皮激素信号的缺失或中断导致气管系统的多种缺陷，包括管腔蛋白沉积减少，背干不均匀扩张和终末分支丢失。我们还揭示了蜕皮激素诱导的基因抛光米（pri）是至关重要的顶端细胞的命运决定背分支。由于prican的过表达可以修复蜕皮激素生物合成障碍引起的缺陷，因此prican是胚胎蜕皮激素信号在气管发生中的主要介质之一。这些结果表明，蜕皮激素及其下游靶蛋白通过调节细胞命运决定在气管发育中起重要作用。
In many animals, progression of developmental stages is temporally controlled by steroid hormones. InDrosophila, the level of ecdysone titer oscillates and developmental stage transitions, such as larval molting and metamorphosis, are induced at each of ecdysone peaks. Ecdysone titer also peaks at the stage of mid‐embryogenesis and the embryonic ecdysone is necessary for morphogenesis of several organs, although the regulatory mechanisms of embryonic organogenesis dependent on ecdysone signaling are still open questions. In this study, we find that absence or interruption of embryonic ecdysone signaling caused multiple defects in the tracheal system, including decrease in luminal protein deposition, uneven dilation of the dorsal trunk and loss of terminal branches. We also reveal that an ecdysone‐inducible genepolished rice(pri) is essential for tip cell fate decision in dorsal branches. As over‐expression ofprican restore the defects caused by disturbance of ecdysone biosynthesis,prifunctions as one of the major mediators of embryonic ecdysone signal in tracheogenesis. These results demonstrate that ecdysone and its downstream targetpriplay essential roles in tracheal development by modulating cell fate decision.