Regulation of the pulsatile releases of luteinizing and follicle-stimulating hormones in ovariectomized hamsters.
Regulation of the pulsatile releases of luteinizing and follicle-stimulating hormones in ovariectomized hamsters.
复制标题
卵巢切除仓鼠黄体生成素和卵泡刺激素脉冲释放的调节。
DOI：
10.1095/biolreprod30.3.628
复制
发表时间：
1984
影响因子：
3.6
通讯作者：
L. Hyland
中科院分区：
文献类型：
--
作者：
S. Chappel;C. Miller;L. Hyland
我们对常用的放射免疫分析(RIA)技术进行了改进，将促性腺激素分析的灵敏度提高了一个数量级，与根据国家脑垂体局提供的说明产生的结果相比。这四种修饰是：a)酶促放射性碘化，b)葡聚糖凝胶和刀豆蛋白A层析纯化放射性标记激素，c)降低第一抗体浓度，d)延长孵育时间。这些方法提高了放射免疫分析的灵敏度，并允许定量测定少量血浆中的黄体生成素(LH)和卵泡刺激素(FSH)水平。我们用这些方法测量了去卵巢仓鼠在各种神经内分泌操作后，黄体生成素和卵泡刺激素的脉冲频率和幅度的变化。儿茶酚胺能神经传递的改变影响黄体生成素释放的频率和幅度，但不影响卵泡刺激素的释放，提示下丘脑负责黄体生成素释放激素(LHRH)介导的黄体生成素释放的机制与调节卵泡刺激素分泌的机制不同。此外，LHRH-垂体相互作用的改变(由注射LHRH抗血清或有效的LHRH激动剂引起)表明，在腺垂体水平上存在单独的控制机制，负责黄体生成素和卵泡刺激素的释放。总而言之，这些研究为神经内分泌对每种促性腺激素的分泌进行复杂和单独的调控提供了进一步的证据。
We have combined for modifications of common radioimmunoassay (RIA) techniques to increase the sensitivity of the gonadotropin assays by an order of magnitude compared with those generated according to the instructions provided by the National Pituitary Agency. The four modifications are: a) enzymatic radioiodination, b) purification of radiolabeled hormones by Sephadex and concanavalin A chromatography, c) reduced first antibody concentration, and d) a prolonged incubation time. These methods increase the sensitivities of the RIAs and allow for the quantitation of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels in small volumes of plasma. We have used these methods to measure the changes in pulse frequency and amplitude of LH and FSH in ovariectomized hamsters after a variety of neuroendocrine manipulations. Alterations in catecholaminergic neurotransmission affect the frequency and amplitude of LH but not FSH release, and suggest that the hypothalamic mechanisms responsible for LH releasing hormone (LHRH)-mediated LH release are distinct from those that regulate FSH secretion. Further, alterations in LHRH-pituitary interactions (elicited by injections of LHRH antisera or a potent LHRH agonist), suggest the existence of separate control mechanisms responsible for LH and FSH release at the level of the adenohypophysis. Combined, these studies provide further evidence for complex and separate neuroendocrine regulatory control over the secretion of each gonadotropin.
去卵巢大鼠的脉动黄体生成素 (LH) 模式：去甲肾上腺素和多巴胺参与 LH 释放激素和 LH 的释放。
影响因子：
4.8
作者：
Negro-Vilar,A;Advis,JP;Ojeda,SR;McCann,SM
通讯作者：
McCann,SM