Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase.
Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase.
复制标题
老挝纹状体中性神经酶的鉴定和生化表征。
DOI：
10.1111/imb.12028
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发表时间：
2013-08
影响因子：
2.6
通讯作者：
Zhu ZR
中科院分区：
文献类型：
--
作者：
Zhou Y;Lin XW;Zhang YR;Huang YJ;Zhang CH;Yang Q;Li HY;Yuan JQ;Cheng JA;Xu R;Mao C;Zhu ZR
神经酰胺酶是一组催化神经酰胺水解产生脂肪酸和鞘氨醇的酶。本文报道了水稻灰飞虱中性神经酰胺酶（lsnCer）基因的克隆和特性分析。通过对灰飞虱（Laodelphax striatellus）的转录组进行测序来鉴定LsnCer。LsnCer是717个氨基酸的蛋白质，预测分子量为79.3 kDa。类似于其他已知的nCD酶，LsnCer具有8.0的最佳pH和37 °C的最佳温度以用于其体外活性。Zn ~（2+）对LsnCer活性有显著的抑制作用，Fe ~（2+）对LsnCer活性有轻微的抑制作用。LsnCer具有广泛的底物特异性，优先选择具有中等酰基链或单不饱和长酰基链的神经酰胺。水稻条纹病毒（RSV）感染或杀虫剂处理显著增加了LsnCer mRNA的表达及其酶活性。纹状体。这些结果表明，LsnCer是一种真正的nCD酶，可能在L.对环境压力的反应。
Ceramidases are a group of enzymes that catalyze hydrolysis of ceramides to generate fatty acid and sphingosine. In this study, we report the cloning and characterization of the rice small brown planthopper Laodelphax striatellus neutral ceramidase (nCDase), LsnCer. LsnCer was identified by sequencing the transcriptome of Laodelphax striatellus. LsnCer is a protein of 717 amino acids with a predicted molecular weight of 79.3 kDa. Similar to other known nCDases, LsnCer has a pH optimum at 8.0 and a temperature optimum at 37 °C for its in vitro activity. LsnCer activity is inhibited by Zn2+ significantly and Fe2+ slightly. LsnCer has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. Infection with the rice strip virus (RSV) or treatment with insecticides significantly increased LsnCer mRNA expression and its enzymatic activity in L. striatellus. These results suggest that LsnCer is a bona fide nCDase that may have a role in adaption of L. striatellus to environmental stresses.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
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作者：
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DOI：
10.1165/rcmb.2003-0424oc
发表时间：
2004-06-01
影响因子：
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作者：
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通讯作者：
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