THE PRODUCT OF THE YEAST UPF1 GENE IS REQUIRED FOR RAPID TURNOVER OF MESSENGER-RNAS CONTAINING A PREMATURE TRANSLATIONAL TERMINATION CODON
THE PRODUCT OF THE YEAST UPF1 GENE IS REQUIRED FOR RAPID TURNOVER OF MESSENGER-RNAS CONTAINING A PREMATURE TRANSLATIONAL TERMINATION CODON
复制标题
DOI：
10.1101/gad.5.12a.2303
复制
发表时间：
1991-12-01
影响因子：
10.5
通讯作者：
CULBERTSON, MR
中科院分区：
文献类型：
--
作者：
LEEDS, P;PELTZ, SW;CULBERTSON, MR
当翻译由于移码或无义突变而过早终止时，mRNA衰变率通常会增加。我们已经确定了一个酵母基因，UPF 1，编码一个反式作用因子，其功能是必要的增强营业额的mRNA含有提前终止密码子。在UPF 1功能缺失的情况下，通常导致mRNA快速衰减的HIS 4或LEU 2基因中的移码或无义突变不能产生这种效果。相反，mRNA以与相应的野生型mRNA相似的速率衰减。在upf 1-菌株中观察到的移码或无义mRNA的稳定似乎不是由于终止信号的增强通读。UPF 1功能的丧失对HIS 4+或LEU 2 + mRNA的积累或稳定性没有影响，这表明UPF 1产物仅响应于提前终止信号而起作用。当我们在UPF 1存在或不存在的情况下检查其他野生型mRNA的积累和稳定性时，包括MAT-α-1，STE 3，ACT 1，PGK 1，PAB 1和URA 3 mRNA，只有URA 3转录物受到影响。在这些和其他结果的基础上，UPF 1产物似乎参与了以前未表征的途径，导致有限类别的酵母转录物的降解。
mRNA decay rates often increase when translation is terminated prematurely due to a frameshift or nonsense mutation. We have identified a yeast gene, UPF1, that codes for a trans-acting factor whose function is necessary for enhanced turnover of mRNAs containing a premature stop codon. In the absence of UPF1 function, frameshift or nonsense mutations in the HIS4 or LEU2 genes that normally cause rapid mRNA decay fail to have this effect. Instead, the mRNAs decay at rates similar to the corresponding wild-type mRNAs. The stabilization of frameshift or nonsense mRNAs observed in upf1- strains does not appear to result from enhanced readthrough of the termination signal. Loss of UPF1 function has no effect on the accumulation or stability of HIS4+ or LEU2+ mRNA, suggesting that the UPF1 product functions only in response to a premature termination signal. When we examined the accumulation and stability of other wild-type mRNAs in the presence or absence of UPF1, including MAT-alpha-1, STE3, ACT1, PGK1, PAB1, and URA3 mRNAs, only the URA3 transcript was affected. On the basis of these and other results, the UPF1 product appears to participate in a previously uncharacterized pathway leading to the degradation of a limited class of yeast transcripts.