Lipid composition of membranes of Escherichia coli by liquid chromatography/tandem mass spectrometry using negative electrospray ionization
Lipid composition of membranes of Escherichia coli by liquid chromatography/tandem mass spectrometry using negative electrospray ionization
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DOI：
10.1002/rcm.3013
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Lange, Catherine M.
中科院分区：
文献类型：
--
作者：
Oursel, Delphine;Loutelier-Bourhis, Corinne;Lange, Catherine M.
建立了一种液相色谱/电喷雾串联质谱（LC/ESI-MS/MS）反相色谱法分析野生型大肠杆菌磷脂的方法。来自[M-H]前体离子的产物离子质谱允许使用诊断产物离子鉴定包括甘油主链上的脂肪酸组成和脂肪酰基位置的单个磷脂物质。因此，该菌株的脂肪酰基位置包括sn-1/sn-2的位置都得到了完全的确定。杆菌此外，使用内标法相对于彼此定量磷脂。版权所有（C）2007约翰威利父子有限公司
A liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method using reversed-phase chromatography was developed for the analysis of phospholipids from bacterial extracts of a wild-type strain of Escherichia coli. Product ion mass spectra from [M-H]precursor ions allowed an identification of individual phospholipid species that includes both fatty acid composition and fatty acyl location on the glycerol backbone using diagnostic product ions. Thus, complete assignment, including sn-1/sn-2 fatty acyl position, was achieved for this strain of E. coli. In addition, the phospholipids were quantified relative to one another using an internal standard method. Copyright (C) 2007 John Wiley & Sons, Ltd.