S100A9 a new marker for monocytic human myeloid-derived suppressor cells
S100A9 a new marker for monocytic human myeloid-derived suppressor cells
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DOI：
10.1111/j.1365-2567.2012.03566.x
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发表时间：
2012-06-01
期刊：
影响因子：
6.4
通讯作者：
Korangy, Firouzeh
中科院分区：
文献类型：
--
作者：
Zhao, Fei;Hoechst, Bastian;Korangy, Firouzeh
髓源性抑制细胞（MDSC）是一种异质性细胞群，在肿瘤进展、炎症和感染期间负调节免疫反应。由于缺乏特异性标记物，关于人类MDSC的数据有限。我们已经鉴定了在CD 14 + HLA-DR-/低MDSC中特异性表达的S100蛋白家族S100 A8、S100 A9和S100 A12的成员。S100 A9染色结合抗CD 14抗体可用于鉴定结肠癌患者全血中的MDSC。与健康对照相比，在结肠癌患者的外周血中观察到CD 14 + S100 A9 high MDSC群体的增加。最后，一氧化氮合酶的表达，MDSC的标志，诱导在CD 14 + S100 A9高脂多糖/干扰素-？刺激.我们建议S100蛋白作为有用的标记物，用于分析和进一步表征人MDSC。
Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of cells that negatively regulate the immune response during tumour progression, inflammation and infection. Only limited data are available on human MDSC because of the lack of specific markers. We have identified members of the S100 protein family S100A8, S100A9 and S100A12 specifically expressed in CD14+ HLA-DR-/low MDSC. S100A9 staining in combination with anti-CD14 could be used to identify MDSC in whole blood from patients with colon cancer. An increase in the population of CD14+ S100A9high MDSC was observed in the peripheral blood from colon cancer patients in comparison with healthy controls. Finally, nitric oxide synthase expression, a hallmark of MDSC, was induced in CD14+ S100A9high upon lipopolysaccharide/interferon-? stimulation. We propose S100 proteins as useful markers for the analysis and further characterization of human MDSC.