Portulaca oleracea L. alleviates liver injury in streptozotocin-induced diabetic mice.
Portulaca oleracea L. alleviates liver injury in streptozotocin-induced diabetic mice.
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马齿苋减轻链脲佐菌素诱导的糖尿病小鼠的肝损伤
DOI：
10.2147/dddt.s121084
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Zheng G;Mo F;Ling C;Peng H;Gu W;Li M;Chen Z
马齿苋是一种分布广泛的肉质草本植物，具有多种药理作用。本研究的目的是评价马齿苋的保护作用。（马齿苋）对链脲佐菌素诱导的小鼠糖尿病的影响。进行口服葡萄糖耐量试验，以评估血糖水平，并记录体重和摄食量。还测量了生化参数抗天冬氨酸转氨酶、丙氨酸转氨酶、胰岛素、甘油三酯、总胆固醇、IL-6、IL-1β和TNFα。苏木精-伊红染色观察肝组织病理变化。Western blot检测肝组织Rho、ROCK 1、ROCK 2、NFκ Bp 65、p-NFκ Bp 65、IκBα、p-IκBα的表达。马齿苋增加体重，减少摄食量。马齿苋还显著降低了血清中葡萄糖、抗天冬氨酸转氨酶、丙氨酸转氨酶、甘油三酯、总胆固醇、IL-6、IL-1β和TNFα的浓度。马齿苋治疗后血清胰岛素升高。此外，马齿苋还能减轻糖尿病小鼠的肝脏病理改变。实验结果表明，与糖尿病小鼠相比，马齿苋能恢复Rho-NFκB信号相关蛋白的水平。推测马齿苋可能通过抑制Rho-NFκB通路，对链脲佐菌素诱导的肝损伤有积极的调节作用。
Purslane is a widespread succulent herb that exhibits various pharmacological effects. The purpose of this study was to evaluate the protective effect of Portulaca oleracea L. (purslane) on streptozotocin-induced diabetes in mice. Oral glucose-tolerance tests were carried out to assess blood glucose levels and body weight and food intake were recorded. The biochemical parameters anti-aspartate aminotransferase, alanine aminotransferase, insulin, triglycerides, total cholesterol, IL-6, IL-1β, and TNFα were also measured. The pathological condition of liver tissues were examined by hematoxylin–eosin staining. Rho, ROCK1, ROCK2, NFκBp65, p-NFκBp65, IκBα, and p-IκBα expression in liver tissue were analyzed by Western blot. Purslane increased body weight and decreased food intake. Purslane also significantly reduced concentrations of glucose, anti-aspartate aminotransferase, alanine aminotransferase, triglycerides, total cholesterol, IL-6, IL-1β, and TNFα in serum. Serum insulin was elevated with purslane treatment. In addition, pathologic liver changes in diabetic mice were also alleviated by purslane. Obtained data revealed that purslane restored the levels of Rho–NFκB signaling-related proteins in comparison with those of diabetic mice. Above all, it can be assumed that purslane might play a positive role in regulating streptozotocin-induced liver injury through suppressing the Rho–NFκB pathway.