PCR detection of Salmonella spp. using primers targeting the quorum sensing gene sdiA
PCR detection of Salmonella spp. using primers targeting the quorum sensing gene sdiA
复制标题
DOI：
10.1111/j.1574-6968.2006.00266.x
复制
发表时间：
2006-06-01
影响因子：
2.1
通讯作者：
Kyriacou, Adamantini
中科院分区：
文献类型：
--
作者：
Halatsi, Konstantia;Oikonomou, Ioannis;Kyriacou, Adamantini
细菌通过化学信号分子相互交流，并与宿主交流。这种现象通常被描述为群体感应。沙门氏菌PCR检测引物的建立。已经设计使用编码LuxR家族的信号受体的sdiA基因作为靶标。PCR产物（274 bp）通过测序确认。对81种非沙门氏菌菌株（代表24种不同的物种）进行了测试，结果为阴性，而总共101种不同血清型的沙门氏菌（155种菌株）对sdiA基因的存在进行了检测。还在人工污染的人类粪便样本中检查了基于sdiA的PCR检测的灵敏度和特异性。在这项研究中，我们证明，群体感应基因可以成功地利用作为诊断标记。
Bacteria communicate with one another and with their host using chemical signalling molecules. This phenomenon is generally described as quorum sensing. A set of primers for PCR detection of Salmonella spp. has been designed using as target the sdiA gene which encodes a signal receptor of the LuxR family. The PCR product (274 bp) was confirmed by sequencing. A number of 81 non-Salmonella strains (representing 24 different species) were tested and gave negative results, while a total of 101 different serotypes of Salmonella (155 strains) tested positive for the presence of the sdiA gene. The sensitivity and specificity of the sdiA-based PCR assay were also checked in artificially contaminated human faecal samples. In this study, we demonstrate that quorum sensing genes can be successfully exploited as diagnostic markers.