Functional Analysis of All AGAMOUS Subfamily Members in Rice Reveals Their Roles in Reproductive Organ Identity Determination and Meristem Determinacy
Functional Analysis of All AGAMOUS Subfamily Members in Rice Reveals Their Roles in Reproductive Organ Identity Determination and Meristem Determinacy
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DOI：
10.1105/tpc.111.087007
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发表时间：
2011-08-01
期刊：
影响因子：
11.6
通讯作者：
Kater, Martin M.
中科院分区：
文献类型：
--
作者：
Dreni, Ludovico;Pilatone, Alessandro;Kater, Martin M.
生殖器官的发育是植物生活史中最重要的步骤之一。利用核心双子叶植物如塔勒水芹和金鱼草的研究表明，属于AGAMOUS（AG）亚家族的MADS结构域转录因子调节雄蕊、心皮和胚珠的身份，并且它们对于花分生组织的决定性很重要。在这里，我们研究了四个水稻（Oryza sativa）AG亚家族成员，MADS3，MADS13，MADS21和MADS58之间的遗传相互作用。我们的数据表明，与以前的报告相比，MADS3和MADS58决定雄蕊和心皮的身份，并与MADS13一起，是重要的花分生组织的决定性。在mads3 mads58双突变体中，我们观察到完全丧失生殖器官的身份和大量积累的浆片在第三和第四个花轮。MADS21是AGL11谱系基因，其表达不限于胚珠。相反，其表达谱与C类基因的表达谱相似。然而，我们的遗传分析表明MADS21在雄蕊、心皮或胚珠身份决定中没有功能。
Reproductive organ development is one of the most important steps in the life cycle of plants. Studies using core eudicot species like thale cress (Arabidopsis thaliana) and snapdragon (Antirrhinum majus) have shown that MADS domain transcription factors belonging to the AGAMOUS (AG) subfamily regulate the identity of stamens, carpels, and ovules and that they are important for floral meristem determinacy. Here, we investigate the genetic interactions between the four rice (Oryza sativa) AG subfamily members, MADS3, MADS13, MADS21, and MADS58. Our data show that, in contrast with previous reports, MADS3 and MADS58 determine stamen and carpel identity and, together with MADS13, are important for floral meristem determinacy. In the mads3 mads58 double mutant, we observed a complete loss of reproductive organ identity and massive accumulation of lodicules in the third and fourth floral whorls. MADS21 is an AGL11 lineage gene whose expression is not restricted to ovules. Instead, its expression profile is similar to those of class C genes. However, our genetic analysis shows that MADS21 has no function in stamen, carpel, or ovule identity determination.