Inductive differentiation of conjunctival goblet cells by γ-secretase inhibitor and construction of recombinant conjunctival epithelium
Inductive differentiation of conjunctival goblet cells by γ-secretase inhibitor and construction of recombinant conjunctival epithelium
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DOI：
10.1016/j.exer.2014.04.001
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Zhou, Qingjun
中科院分区：
文献类型：
--
作者：
Tian, Le;Qu, Mingli;Zhou, Qingjun
γ-分泌酶抑制剂已显示促进肠杯状细胞分化。我们现在证明，在体外添加γ-分泌酶抑制剂的人结膜上皮细胞的培养显着促进结膜杯状细胞的分化与典型的液滴样表型，阳性高碘酸希夫和杯状细胞特异性Muc 5Ac，细胞角蛋白7和Hessopomatia凝集素凝集素染色。此外，局部应用γ-分泌酶抑制剂促进小鼠结膜杯状细胞在体内的分化。此外，Notch靶基因HES-1的表达在结膜杯状细胞分化过程中下调。此外，我们发现羊膜上的重组结膜上皮与正常结膜上皮相比，杯状细胞密度较低，位置异常，在最终诱导时添加γ-分泌酶抑制剂可改善这些情况。(C)2014爱思唯尔有限公司版权所有。
gamma-secretase inhibitor has been shown to promote intestinal goblet cell differentiation. We now demonstrated that the in vitro addition of gamma-secretase inhibitor in the culture of human conjunctival epithelial cells significantly promoted the differentiation of conjunctival goblet cells with typical droplet-like phenotype, positive periodic acid-Schiff and goblet cell-specific Muc5Ac, cytokeratin 7 and Helix pomatia agglutinin lectin staining. Moreover, topical application of gamma-secretase inhibitor promoted the differentiation of mouse conjunctival goblet cells in vivo. Furthermore, the expression of Notch target gene HES-1 was down-regulated during the differentiation of conjunctival goblet cells. In addition, we found that the recombinant conjunctival epithelium on amniotic membrane showed less goblet cell density and abnormal location when compared with normal conjunctival epithelium, which were improved by the addition of gamma-secretase inhibitor in the final induction. (C) 2014 Elsevier Ltd. All rights reserved.