Updates to the sporadic knowledge on microsporidian infections in groundwater amphipods (Crustacea, Amphipoda, Niphargidae)
Updates to the sporadic knowledge on microsporidian infections in groundwater amphipods (Crustacea, Amphipoda, Niphargidae)
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DOI：
10.3897/subtbiol.33.48633
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发表时间：
2020-02-13
影响因子：
1.1
通讯作者：
Weigand, Alexander M.
中科院分区：
文献类型：
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作者：
Grabner, Daniel;Weber, Dieter;Weigand, Alexander M.
对微孢子虫属Niphargus、Niphargellus、Microniphargus和Crangonix属的19个地下水物种的69个样本进行了遗传筛选。样本大多来自依赖地下水的泉水环境(71%)、天然洞穴(9%)和人工洞穴/隧道(13%)。通过形态和/或分子数据鉴定双足类寄主，而微孢子虫寄生虫是通过针对rRNA小亚基基因片段的遗传筛选实验来鉴定的。从13个寄主标本(谢伦贝氏小卷蛾；B、F和G世系；阿氏小卷蛾)中发现。特别是，斯氏新月球虫经常感染杜氏杜氏菌和一种新的、可能具有奈氏杆菌特异性的微孢子虫。我们的结果进一步阐明了在地下水、圆足类和地下动物中寄生微孢子虫的多样性和特异性。
A set of 69 specimens from 19 groundwater species of the genera Niphargus, Niphargellus, Microniphargus and Crangonyx was genetically screened for microsporidian infections. Samples mostly originated from groundwater-dependent spring environments (71%), natural caves (9%) and artificial caverns/tunnels (13%). Amphipod hosts were identified by morphology and/or molecular data, whereas microsporidian parasites were characterised by a genetic screening assay targeting a section of the small subunit rRNA gene.Five microsporidian species (Dictyocoela duebenum; Nosema sp.; Hyperspora aquatica and two undescribed Microsporidium spp.) were revealed from 13 host specimens (Niphargus schellenbergi; N. aquilex lineages B, F and G; Niphargellus arndti). In particular N. schellenbergi was frequently infected with D. duebenum as well as a new and potentially niphargid-specific Nosema sp. identified in Niphargellus arndti.Our results shed further light on the still largely unknown diversity and specificity of microsporidian parasites in groundwater amphipods and subterranean animals in general.