Optimal poly (3-hydroxybutyrate/3-hydroxyvalerate) biosynthesis by fermentation liquid from primary and waste activated sludge
Optimal poly (3-hydroxybutyrate/3-hydroxyvalerate) biosynthesis by fermentation liquid from primary and waste activated sludge
复制标题
初生污泥和废活性污泥发酵液优化聚（3-羟基丁酸酯/3-羟基戊酸酯）生物合成
DOI：
10.1080/09593330.2014.882993
复制
发表时间：
2014-07-18
影响因子：
2.8
通讯作者：
Huo, Mingxin
中科院分区：
文献类型：
--
作者：
Chen, Yinguang;Li, Mu;Huo, Mingxin
以初沉污泥（PS）和剩余污泥（WAS）发酵液为碳源，研究了活性污泥发酵生产聚（3-羟基丁酸/3-羟基戊酸）（PHBV）的工艺条件。首先，确定用于具有所需羟基戊酸酯（HV）级分的PHBV合成的乙酸与丙酸（乙酸/丙酸）的合适浓度和比率。然后，优化了从PS/WAS混合物生产具有所需乙酸/丙酸的发酵液的条件。最后以优化后的发酵液为碳源，采用好氧补料和出料工艺合成PHBV。污泥中PHBV含量达到65.5%，HV单元含量为47.8%（mol-C计），每升驯化污泥相应的聚羟基脂肪酸酯产量为1.44 g/L。这是首次报道的以活性污泥为微生物、污泥发酵液为碳源的PHBV生物合成最佳条件。基于聚合酶链反应的454焦磷酸测序分析显示，Thauera菌株是PHBV生物合成系统中的优势种。
In this paper the production of poly (3-hydroxybutyrate/3-hydroxyvalerate) (PHBV) with activated sludge was investigated by using the fermentation liquid from primary sludge (PS) and waste activated sludge (WAS) as carbon source. First, the suitable concentration and ratio of acetic to propionic (acetic/propionic) for PHBV synthesis with desired hydroxyvalerate (HV) fraction was determined. Then, the conditions for producing fermentation liquid with the required acetic/propionic from the PS/WAS mixture were optimized. Finally, this optimized fermentation liquid was used as the carbon source for PHBV synthesis by the aerobic feeding and discharge process. The PHBV content in the sludge reached 65.5%, with 47.8% (mol-C based) of HV unit content, and the corresponding polyhydroxyalkanoates yield per litre of acclimated sludge was 1.44 g/L. This was the first optimal PHBV biosynthesis reported by using activated sludge as the microbe and sludge fermentation liquid as the carbon source. Polymerase chain reaction-based 454 pyrosequencing analysis revealed that Thauera strains were the predominant species in the PHBV biosynthesis system.