Therapeutic efficacy of human umbilical cord mesenchymal stem cells transplantation against renal ischemia/reperfusion injury in rats
Therapeutic efficacy of human umbilical cord mesenchymal stem cells transplantation against renal ischemia/reperfusion injury in rats
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DOI：
10.1016/j.tice.2017.04.006
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发表时间：
2017-06-01
期刊：
影响因子：
2.6
通讯作者：
Mohsen, Heba
中科院分区：
文献类型：
--
作者：
Fahmy, Sohair R.;Soliman, Amel M.;Mohsen, Heba
背景：急性肾损伤（阿基）是一种常见的临床问题，迫切需要开发新的治疗策略。本研究旨在探讨人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，HUC-MSCs）移植对大鼠肾缺血再灌注损伤（ischemia/reperfusion injury，IRI）的治疗作用。结果：HUC-MSCs能明显改善I/R所致的肾损伤，并能降低血清肌酐、尿素和尿酸水平，增强肾功能。HUC-MSCs的抗氧化作用是通过显著降低总丙二醛（MDA）和显著增加还原型谷胱甘肽（GSH）、过氧化氢酶（CAT）和谷胱甘肽-S-转移酶（GST）水平来介导的。结论：HUC-MSCs可能是一种有效的抗肾IRI的治疗剂。记录数据显示HUC-MSCs的肾功能和尿白蛋白较IRI组改善，HUC-MSCs通过清除自由基和支持抗氧化酶而具有积极的抗氧化功效。（C）2017爱思唯尔有限公司版权所有。
Background: Acute kidney injury (AKI) is a common clinical problem raising the urgent needs to develop new strategies for treatment. The present study investigated the therapeutic potential of human umbilical cord - mesenchymal stem cells (HUC-MSCs) transplantation against renal ischemia/reperfusion injury (IRI) in rats.Methods: Twenty four male Wistar rats were assigned into two main groups, sham group (control group) and I/R group. I/R group was injected in the tail vein with either phosphate buffer saline (PBS) or HUC-MSCs.Results: The HUC-MSCs improved kidney injury induced by I/R as demonstrated by enhancement of the kidney function via decreasing serum levels of creatinine, urea and uric acid. The therapeutic efficacy of HUC-MSCs were found to be mediated through anti-oxidant activity as indicated by significant reduction in total malondialdehyde (MDA) and significant increment in the levels of reduced glutathione (GSH), catalase (CAT) and glutathione-S-transferase (GST).Conclusion: The present work suggests that HUC-MSCs may be an effective therapeutic agent against renal IRI. The recorded data showed improvement of renal functions and urine albumin in HUC-MSCs than IRI group with positive antioxidant efficacy of HUC-MSCs through scavenging free radicals and supporting the antioxidant enzymes. (C)2017 Elsevier Ltd. All rights reserved.