Imaging with organic indicators and high-speed charge-coupled device cameras in neurons: some applications where these classic techniques have advantages.
Imaging with organic indicators and high-speed charge-coupled device cameras in neurons: some applications where these classic techniques have advantages.
复制标题
在神经元中使用有机指示剂和高速电荷耦合器件相机进行成像：这些经典技术在一些应用中具有优势。
DOI：
10.1117/1.nph.2.2.021005
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发表时间：
2015
期刊：
影响因子：
5.3
通讯作者：
Zecevic,Dejan
中科院分区：
文献类型：
--
作者：
Ross,WilliamN;Miyazaki,Kenichi;Popovic,MarkoA;Zecevic,Dejan
动态钙和电压成像是现代细胞神经科学的主要工具。自40多年前开始使用以来，指示器、显微镜、成像系统和计算机都有了重大改进。虽然前沿研究已经趋向于使用基因编码的钙或电压指标，双光子显微镜和体内修复，但值得注意的是，一些问题仍然可能是最好的方法，使用更经典的方法和准备。在这篇综述中，我们突出了几个例子，在神经元中的电荷耦合器件（CCD）成像和经典的有机指标的组合揭示了信息，迄今为止，使用更现代的系统比结果更翔实。这些实验利用了最好的CCD相机的高帧速率、灵敏度和空间集成度。这些相机可以响应有机电压和钙指标的更快动力学，这密切反映了潜在的细胞事件的快速动力学。
Dynamic calcium and voltage imaging is a major tool in modern cellular neuroscience. Since the beginning of their use over 40 years ago, there have been major improvements in indicators, microscopes, imaging systems, and computers. While cutting edge research has trended toward the use of genetically encoded calcium or voltage indicators, two-photon microscopes, andin vivopreparations, it is worth noting that some questions still may be best approached using more classical methodologies and preparations. In this review, we highlight a few examples in neurons where the combination of charge-coupled device (CCD) imaging and classical organic indicators has revealed information that has so far been more informative than results using the more modern systems. These experiments take advantage of the high frame rates, sensitivity, and spatial integration of the best CCD cameras. These cameras can respond to the faster kinetics of organic voltage and calcium indicators, which closely reflect the fast dynamics of the underlying cellular events.