Mitochondrial DNA mutations in exhaled breath condensate of patients with lung cancer
Mitochondrial DNA mutations in exhaled breath condensate of patients with lung cancer
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DOI：
10.1016/j.rmed.2013.02.007
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发表时间：
2013-06-01
影响因子：
4.3
通讯作者：
Thomas, Paul S.
中科院分区：
文献类型：
--
作者：
Ai, Sylvia Si Yang;Hsu, Kenneth;Thomas, Paul S.
简介：肺癌是全球癌症死亡率的主要原因。非侵入性收集的生物流体，如呼出气冷凝液（EBC）提供了一种潜在的采样介质，以检测和研究与肿瘤发生有关的病理变化。线粒体DNA的变化与癌变过程有关。因此，EBC中线粒体变化的检测可以扩大我们对肺癌发生的理解，以及为未来的研究确定特定的标志物。方法：收集EBC和唾液从新诊断的肺癌和对照组的受试者在横断面研究。使用PCR测序方法分析EBC和唾液的线粒体DNA D环变化。将获得的序列与配对的唾液DNA和修订的剑桥参考序列（rCRS）进行比较，以确定体细胞突变，并对组间突变的定量和定性差异进行分析。结果：共招募了25例受试者（9例NSCLC患者，10例吸烟者/戒烟者和6例非吸烟者）。与对照组相比，肺癌组的D环突变率显著升高（吸烟者/戒烟者为7 vs. 3.5，非吸烟者为7 vs. 4，p = 0.034）。结论：线粒体DNA突变在肺癌患者EBC中更为常见。这表明这些过程与肺癌的致癌作用有关，可能是该疾病的标志物。(C)2013爱思唯尔有限公司保留所有权利。
Introduction: Lung cancer is a leading cause of cancer mortality worldwide. Non-invasively collected biofluids such as exhaled breath condensate (EBC) present a potential sampling medium to detect and study pathological changes implicated in tumourigenesis. Mitochondrial DNA changes have been implicated in the carcinogenesis process. Consequently, the detection of mitochondrial changes in EBC could expand our understanding of lung carcinogenesis as well as identifying specific markers for future studies.Methods: EBC and saliva was collected from newly diagnosed subjects with lung cancer and control subjects in a cross-sectional study. The EBC and saliva was analysed for mitochondrial DNA D-loop changes using a PCR sequencing approach. The sequences obtained were compared to paired salivary DNA and the revised Cambridge Reference Sequence (rCRS) to identify somatic mutations, and quantitative and qualitative differences in mutations were analysed between groups.Results: A total of 25 subjects (9 NSCLC patients, 10 smokers/ex-smokers and 6 non-smokers) were recruited. A significantly elevated D-loop mutation rate in the lung cancer group compared to the control groups was present (7 vs 3.5 for smokers/ex-smokers, and 7 vs. 4 for non-smokers, p = 0.034). The recognised mutation T16217C showed specificity for lung cancer.Conclusions: Mitochondrial DNA mutations are more common in the EBC of patients with lung cancer. This suggests that these processes are associated with the carcinogenesis of lung cancer and may be a marker of the disease. (C) 2013 Elsevier Ltd. All rights reserved.