Aggregated Silver Nanoparticles Based Surface-Enhanced Raman Scattering Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Protein Biomarkers and Small Molecules
Aggregated Silver Nanoparticles Based Surface-Enhanced Raman Scattering Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Protein Biomarkers and Small Molecules
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基于聚集银纳米颗粒的表面增强拉曼散射酶联免疫吸附测定，用于蛋白质生物标志物和小分子的超灵敏检测
DOI：
10.1021/acs.analchem.5b01011
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发表时间：
2015-06-02
影响因子：
7.4
通讯作者：
Tang, Yong
中科院分区：
文献类型：
--
作者：
Liang, Jiajie;Liu, Hongwu;Tang, Yong
降低检测限对于医学诊断、环境监测和食品安全法规所需的生物测定的设计至关重要。目前标准的、基于颜色的分析物检测的灵敏度限制了酶联免疫吸附测定（ELISA）在研究和临床诊断中的进一步使用。在这里;我们展示了一种新的方法，该方法使用拉曼信号作为ELISA的信号产生系统，并将表面增强拉曼散射（Sers）与银纳米颗粒聚集相结合，用于超灵敏的分析物检测。ELISA的酶标记控制拉曼荧光标记的银纳米颗粒的溶解：通过过氧化氢，并在分析物存在时产生强拉曼信号。使用该测定法，在全血清和尿液中分别检测到10（-9）和10（-6）ng/mL超低浓度的前列腺特异性抗原（PSA）和肾上腺兴奋剂莱克多巴胺（Rac）。本文提出的方法可应用于PSA和Rac等其他分子的检测。
Lowering the detection limit is critical to the design of,, bioassays required for medical diagnostics environmental monitoring, and food, safety regulations. The current sensitivity of standard, color-based analyte detection limits the further use Of enzyme-linked immunosorbent, assays (ELISAs) in research and clinical diagnoses. Here; we demonstrate a novel method that uses the Raman signal as the signal-generating system of an ELISA and combines surface-enhanced Raman scattering (SERS) with, silver nanoparticles aggregation for ultrasensitive analyte detection. The enzyme label of the ELISA controls the dissolution of Raman reporter-labeled silver nanoparticles: through hydrogen peroxide and generates strong Raman signal when the analyte is present. Using this assay, prostate-specific antigen (PSA) and the adrenal stimulant ractopamine (Rac), were detected in whole serum: and urine at the ultralow concentrations of 10(-9) and 10(-6) ng/mL, respectively. The methodology proposed here could potentially be applied to other molecules detection as well as PSA and Rac.