Living Materials Based Dynamic Information Encryption via Light-Inducible Bacterial Biosynthesis of Quantum Dots
Living Materials Based Dynamic Information Encryption via Light-Inducible Bacterial Biosynthesis of Quantum Dots
复制标题
DOI：
10.1002/anie.202315251
复制
发表时间：
2023-12-12
影响因子：
16.6
通讯作者：
Wu，Yihan
中科院分区：
文献类型：
--
作者：
Niu，Luqi;Yu，Lin;Wu，Yihan
微生物生物合成作为制备量子点（QDs）的一种替代方法，因其可以在温和和环境友好的条件下进行，与传统的化学和物理合成方法不同而受到关注。然而，目前还没有方法可以使用外部刺激选择性地控制群体内一小部分微生物的这种生物合成过程。在本研究中，我们利用光基因工程的大肠杆菌（E.）实现了对量子点微生物生物合成的精确和选择性控制。杆菌)。recombinantE。设计表达smCSE酶的大肠杆菌，在eLightOn系统的调控下，可被蓝光激活。smCSE酶利用L -半胱氨酸和Cd2+作为底物形成CdS量子点。该系统能够在水凝胶中以精确的模式光诱导细菌生物合成量子点，用于信息加密。随着生物合成的进行，量子点的光学特性发生了变化，使得含有重组的生命材料成为可能。Colito显示时间依赖模式，读取后自毁。与使用荧光量子点油墨的静态加密相比，基于活体材料的动态信息加密提供了更高的安全性。
Microbial biosynthesis, as an alternative method for producing quantum dots (QDs), has gained attention because it can be conducted under mild and environmentally friendly conditions, distinguishing it from conventional chemical and physical synthesis approaches. However, there is currently no method to selectively control this biosynthesis process in a subset of microbes within a population using external stimuli. In this study, we have attained precise and selective control over the microbial biosynthesis of QDs through the utilization of an optogenetically engineeredEscherichia coli(E. coli). The recombinantE. coliis designed to express smCSE enzyme, under the regulation of eLightOn system, which can be activated by blue light. The smCSE enzymes use L‐cysteine and Cd2+as substrates to form CdS QDs. This system enables light‐inducible bacterial biosynthesis of QDs in precise patterns within a hydrogel for information encryption. As the biosynthesis progresses, the optical characteristics of the QDs change, allowing living materials containing the recombinantE. colito display time‐dependent patterns that self‐destruct after reading. Compared to static encryption using fluorescent QD inks, dynamic information encryption based on living materials offers enhanced security.