Circulating Nucleic Acids in Plasma and Serum: An Overview
Circulating Nucleic Acids in Plasma and Serum: An Overview
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DOI：
10.1111/j.1749-6632.2001.tb03858.x
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发表时间：
2001-09
影响因子：
5.2
通讯作者：
Y. Lo
中科院分区：
文献类型：
--
作者：
Y. Lo
摘要：最近对血浆和血清中的核酸的兴趣为分子诊断开辟了许多新的研究领域和新的可能性。在肿瘤学中，在癌症患者的血浆/血清中发现了肿瘤源性遗传变化、表观遗传改变和病毒核酸。这些发现对许多类型的恶性肿瘤的检测、监测和诊断具有重要意义。在产前诊断中，母体血浆和血清中胎儿DNA的发现提供了用于分析的胎儿遗传物质的非侵入性来源。这一发展对实现无创产前诊断具有重要意义，并为妊娠相关疾病的监测提供了新方法。血浆DNA技术最近还应用于器官移植、创伤后监测和感染因子检测领域。未来的研究领域包括血浆中的循环RNA和血浆核酸的释放、清除和可能功能的生物学阐明。
Abstract: The recent interest in nucleic acids in plasma and serum has opened up numerous new areas of investigation and new possibilities for molecular diagnosis. In oncology, tumor‐derived genetic changes, epigenetic alterations, and viral nucleic acids have been found in the plasma/serum of cancer patients. These findings have important implications for the detection, monitoring, and prognostication of many types of malignancies. In prenatal diagnosis, the discovery of fetal DNA in maternal plasma and serum has provided a noninvasive source of fetal genetic material for analysis. This development has important implications for the realization of noninvasive prenatal diagnosis and has provided new methods for the monitoring of pregnancy‐associated disorders. Plasma DNA technology has also found recent applications in the fields of organ transplantation, posttrauma monitoring, and infectious agent detection. Future areas of study include circulating RNA in plasma and the elucidation of the biology of release, clearance, and possible functionality of plasma nucleic acids.